{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T04:40:24Z","timestamp":1781584824245,"version":"3.54.5"},"reference-count":47,"publisher":"National Academy of Sciences","issue":"13","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,3,27]]},"abstract":"<jats:p>\n                    The complete genome sequence of\n                    <jats:italic>Geobacillus thermodenitrificans<\/jats:italic>\n                    NG80-2, a thermophilic bacillus isolated from a deep oil reservoir in Northern China, consists of a 3,550,319-bp chromosome and a 57,693-bp plasmid. The genome reveals that NG80-2 is well equipped for adaptation into a wide variety of environmental niches, including oil reservoirs, by possessing genes for utilization of a broad range of energy sources, genes encoding various transporters for efficient nutrient uptake and detoxification, and genes for a flexible respiration system including an aerobic branch comprising five terminal oxidases and an anaerobic branch comprising a complete denitrification pathway for quick response to dissolved oxygen fluctuation. The identification of a nitrous oxide reductase gene has not been previously described in Gram-positive bacteria. The proteome further reveals the presence of a long-chain alkane degradation pathway; and the function of the key enzyme in the pathway, the long-chain alkane monooxygenase LadA, is confirmed by\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    experiments. The thermophilic soluble monomeric LadA is an ideal candidate for treatment of environmental oil pollutions and biosynthesis of complex molecules.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0609650104","type":"journal-article","created":{"date-parts":[[2007,3,19]],"date-time":"2007-03-19T20:36:00Z","timestamp":1174336560000},"page":"5602-5607","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":354,"title":["Genome and proteome of long-chain alkane degrading\n                    <i>Geobacillus thermodenitrificans<\/i>\n                    NG80-2 isolated from a deep-subsurface oil reservoir"],"prefix":"10.1073","volume":"104","author":[{"given":"Lu","family":"Feng","sequence":"first","affiliation":[{"name":"*TEDA School of Biological Sciences and Biotechnology and"},{"name":"Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, 23 Hongda Street, Tianjin Economic-Technological Development Area (TEDA), 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