{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T04:04:15Z","timestamp":1792296255037,"version":"4.4.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"18","license":[{"start":{"date-parts":[[2007,10,23]],"date-time":"2007-10-23T00:00:00Z","timestamp":1193097600000},"content-version":"vor","delay-in-days":175,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:p>\n                    The signaling molecule nitric oxide (NO), first described as endothelium-derived relaxing factor (EDRF), acts as physiological activator of NO-sensitive guanylyl cyclase (NO-GC) in the cardiovascular, gastrointestinal, and nervous systems. Besides NO-GC, other NO targets have been proposed; however, their particular contribution still remains unclear. Here, we generated mice deficient for the \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    subunit of NO-GC, which resulted in complete loss of the enzyme. GC-KO mice have a life span of 3\u20134 weeks but then die because of intestinal dysmotility; however, they can be rescued by feeding them a fiber-free diet. Apparently, NO-GC is absolutely vital for the maintenance of normal peristalsis of the gut. GC-KO mice show a pronounced increase in blood pressure, underlining the importance of NO in the regulation of smooth muscle tone\n                    <jats:italic>in vivo<\/jats:italic>\n                    . The lack of an NO effect on aortic relaxation and platelet aggregation confirms NO-GC as the only NO target regulating these two functions, excluding cGMP-independent mechanisms. Our knockout model completely disrupts the NO\/cGMP signaling cascade and provides evidence for the unique role of NO-GC as NO receptor.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0609778104","type":"journal-article","created":{"date-parts":[[2007,4,24]],"date-time":"2007-04-24T11:46:17Z","timestamp":1177415177000},"page":"7699-7704","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":207,"title":["Fatal gastrointestinal obstruction and hypertension in mice lacking nitric oxide-sensitive guanylyl cyclase"],"prefix":"10.1073","volume":"104","author":[{"given":"Andreas","family":"Friebe","sequence":"first","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Medizinische Fakult\u00e4t, Ruhr-Universit\u00e4t Bochum, 44780 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evanthia","family":"Mergia","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Medizinische Fakult\u00e4t, Ruhr-Universit\u00e4t Bochum, 44780 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Dangel","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Medizinische Fakult\u00e4t, Ruhr-Universit\u00e4t Bochum, 44780 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Lange","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Medizinische Fakult\u00e4t, Ruhr-Universit\u00e4t Bochum, 44780 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Koesling","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Pharmakologie und Toxikologie, Medizinische Fakult\u00e4t, Ruhr-Universit\u00e4t Bochum, 44780 Bochum, 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