{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:02:04Z","timestamp":1791291724258,"version":"4.1.0"},"reference-count":37,"publisher":"National Academy of Sciences","issue":"3","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,1,16]]},"abstract":"<jats:p>\n                    Wiskott\u2013Aldrich syndrome protein (WASP) is in a complex with WASP-interacting protein (WIP). WASP levels, but not mRNA levels, were severely diminished in T cells from WIP\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice and were increased by introduction of WIP in these cells. The WASP binding domain of WIP was shown to protect WASP from degradation by calpain\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Treatment with the proteasome inhibitors MG132 and bortezomib increased WASP levels in T cells from WIP\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice and in T and B lymphocytes from two WAS patients with missense mutations (R86H and T45M) that disrupt WIP binding. The calpain inhibitor calpeptin increased WASP levels in activated T and B cells from the WASP patients, but not in primary T cells from the patients or from WIP\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice. Despite its ability to increase WASP levels proteasome inhibition did not correct the impaired IL-2 gene expression and low F-actin content in T cells from the R86H WAS patient. These results demonstrate that WIP stabilizes WASP and suggest that it may also be important for its function.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0610275104","type":"journal-article","created":{"date-parts":[[2007,1,9]],"date-time":"2007-01-09T23:37:48Z","timestamp":1168385868000},"page":"926-931","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":150,"title":["WIP is a chaperone for Wiskott\u2013Aldrich syndrome protein (WASP)"],"prefix":"10.1073","volume":"104","author":[{"given":"Miguel A.","family":"de la Fuente","sequence":"first","affiliation":[{"name":"*Division of Immunology, Children's Hospital Boston, and"},{"name":"Department of Pediatrics, Harvard Medical School, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoji","family":"Sasahara","sequence":"additional","affiliation":[{"name":"*Division of Immunology, Children's Hospital Boston, and"},{"name":"Department of 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