{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T06:33:05Z","timestamp":1791354785476,"version":"4.3.1"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2001,3,27]]},"abstract":"<jats:p>\n                    The complete genome sequence of\n                    <jats:italic>Caulobacter\n crescentus<\/jats:italic>\n                    was determined to be 4,016,942 base pairs in a\n single circular chromosome encoding 3,767 genes. This organism, which\n grows in a dilute aquatic environment, coordinates the cell division\n cycle and multiple cell differentiation events. With the annotated\n genome sequence, a full description of the genetic network that\n controls bacterial differentiation, cell growth, and cell cycle\n progression is within reach. Two-component signal transduction proteins\n are known to play a significant role in cell cycle progression. Genome\n analysis revealed that the\n                    <jats:italic>C. crescentus<\/jats:italic>\n                    genome encodes\n a significantly higher number of these signaling proteins (105) than\n any bacterial genome sequenced thus far. Another regulatory mechanism\n involved in cell cycle progression is DNA methylation. The occurrence\n of the recognition sequence for an essential DNA methylating enzyme\n that is required for cell cycle regulation is severely limited and\n shows a bias to intergenic regions. The genome contains multiple\n clusters of genes encoding proteins essential for survival in a\n nutrient poor habitat. Included are those involved in chemotaxis, outer\n membrane channel function, degradation of aromatic ring compounds, and\n the breakdown of plant-derived carbon sources, in addition to many\n extracytoplasmic function sigma factors, providing the organism with\n the ability to respond to a wide range of environmental fluctuations.\n                    <jats:italic>C. crescentus<\/jats:italic>\n                    is, to our knowledge, the first\n free-living \u03b1-class proteobacterium to be sequenced and will serve as\n a foundation for exploring the biology of this group of bacteria, which\n includes the obligate endosymbiont and human pathogen\n                    <jats:italic>Rickettsia\n prowazekii<\/jats:italic>\n                    , the plant pathogen\n                    <jats:italic>Agrobacterium\n tumefaciens<\/jats:italic>\n                    , and the bovine and human pathogen\n                    <jats:italic>Brucella\n abortus<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.061029298","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:44:19Z","timestamp":1027680259000},"page":"4136-4141","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":458,"title":["Complete genome sequence of\n                    <i>Caulobacter crescentus<\/i>"],"prefix":"10.1073","volume":"98","author":[{"given":"William C.","family":"Nierman","sequence":"first","affiliation":[{"name":"The Institute for Genomic Research, 9712 Medical\r Center Drive, Rockville, MD 20850; Department of\r Developmental Biology, Beckman Center, Stanford University School\r of Medicine, Stanford, CA 94305; Department of\r Biochemistry, Imperial College of Science, Technology and Medicine,\r London SW7 2AY, United Kingdom; Department of Molecular\r Biology, Princeton University, Princeton, NJ 08544;\r Department of Biology, University of Michigan, 830 North\r University, Ann Arbor, MI 48109-1048; Biology..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara V.","family":"Feldblyum","sequence":"additional","affiliation":[{"name":"The Institute for Genomic Research, 9712 Medical\r Center Drive, Rockville, MD 20850; Department of\r Developmental Biology, Beckman Center, Stanford University School\r of Medicine, Stanford, CA 94305; Department of\r Biochemistry, Imperial College of Science, Technology and Medicine,\r London SW7 2AY, United Kingdom; Department of Molecular\r Biology, Princeton University, Princeton, NJ 08544;\r Department of Biology, University of Michigan, 830 North\r University, Ann Arbor, MI 48109-1048; Biology..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael T.","family":"Laub","sequence":"additional","affiliation":[{"name":"The Institute for Genomic Research, 9712 Medical\r Center Drive, Rockville, MD 20850; Department of\r Developmental Biology, Beckman Center, Stanford University School\r of Medicine, Stanford, CA 94305; Department of\r Biochemistry, Imperial College of Science, Technology and Medicine,\r London SW7 2AY, United Kingdom; Department of Molecular\r Biology, Princeton University, Princeton, NJ 08544;\r Department of Biology, University of Michigan, 830 North\r University, Ann Arbor, MI 48109-1048; Biology..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian T.","family":"Paulsen","sequence":"additional","affiliation":[{"name":"The Institute for Genomic Research, 9712 Medical\r Center Drive, Rockville, MD 20850; Department of\r Developmental Biology, Beckman Center, Stanford University School\r of Medicine, Stanford, CA 94305; 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K.","family":"Alley","sequence":"additional","affiliation":[{"name":"The Institute for Genomic Research, 9712 Medical\r Center Drive, Rockville, MD 20850; Department of\r Developmental Biology, Beckman Center, Stanford University School\r of Medicine, Stanford, CA 94305; Department of\r Biochemistry, Imperial College of Science, Technology and Medicine,\r London SW7 2AY, United Kingdom; Department of Molecular\r Biology, Princeton University, Princeton, NJ 08544;\r Department of Biology, University of Michigan, 830 North\r University, Ann Arbor, MI 48109-1048; Biology..."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Ohta","sequence":"additional","affiliation":[{"name":"The Institute for Genomic Research, 9712 Medical\r Center Drive, Rockville, MD 20850; Department of\r Developmental Biology, Beckman Center, Stanford University School\r of Medicine, Stanford, CA 94305; Department of\r Biochemistry, Imperial College of Science, Technology and Medicine,\r London SW7 2AY, United Kingdom; 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