{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T11:10:55Z","timestamp":1793445055706,"version":"4.5.0"},"reference-count":31,"publisher":"National Academy of Sciences","issue":"36","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,9,4]]},"abstract":"<jats:p>\n                    Autophagy is a regulated lysosomal degradation process that involves autophagosome formation and transport. Although recent evidence indicates that basal levels of autophagy protect against neurodegeneration, the exact mechanism whereby this occurs is not known. By using conditional knockout mutant mice, we report that neuronal autophagy is particularly important for the maintenance of local homeostasis of axon terminals and protection against axonal degeneration. We show that specific ablation of an essential autophagy gene,\n                    <jats:italic>Atg7<\/jats:italic>\n                    , in Purkinje cells initially causes cell-autonomous, progressive dystrophy (manifested by axonal swellings) and degeneration of the axon terminals. Consistent with suppression of autophagy, no autophagosomes are observed in these dystrophic swellings, which is in contrast to accumulation of autophagosomes in the axonal dystrophic swellings under pathological conditions. Axonal dystrophy of mutant Purkinje cells proceeds with little sign of dendritic or spine atrophy, indicating that axon terminals are much more vulnerable to autophagy impairment than dendrites. This early pathological event in the axons is followed by cell-autonomous Purkinje cell death and mouse behavioral deficits. Furthermore, ultrastructural analyses of mutant Purkinje cells reveal an accumulation of aberrant membrane structures in the axonal dystrophic swellings. Finally, we observe double-membrane vacuole-like structures in wild-type Purkinje cell axons, whereas these structures are abolished in mutant Purkinje cell axons. Thus, we conclude that the autophagy protein Atg7 is required for membrane trafficking and turnover in the axons. Our study implicates impairment of axonal autophagy as a possible mechanism for axonopathy associated with neurodegeneration.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0701311104","type":"journal-article","created":{"date-parts":[[2007,8,28]],"date-time":"2007-08-28T22:44:04Z","timestamp":1188341044000},"page":"14489-14494","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":572,"title":["Essential role for autophagy protein Atg7 in the maintenance of axonal homeostasis and the prevention of axonal degeneration"],"prefix":"10.1073","volume":"104","author":[{"given":"Masaaki","family":"Komatsu","sequence":"first","affiliation":[{"name":"*Laboratory of Frontier Science, Tokyo Metropolitan Institute of Medical Science, Bunkyo-ku, Tokyo 113-8613, Japan;"},{"name":"Department of Biochemistry, Juntendo University School of Medicine, Bunkyo-ku, Tokyo 113-8421, Japan;"},{"name":"Precursory Research for Embryonic Science and Technology, Japan Science and Technology Corporation, Kawaguchi 332-0012, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Neuroscience, Mount Sinai School of Medicine, New York, NY 10029;"},{"name":"Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University, New York, NY 10065"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gay R.","family":"Holstein","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Neuroscience, Mount Sinai School of Medicine, New York, NY 10029;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"Victor L.","family":"Friedrich","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Neuroscience, Mount Sinai School of Medicine, New York, NY 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