{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T10:24:54Z","timestamp":1791023094503,"version":"4.1.0"},"reference-count":51,"publisher":"National Academy of Sciences","issue":"27","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,7,3]]},"abstract":"<jats:p>\n                    Testis-derived testosterone has been recognized as the key factor for morphogenesis of the Wolffian duct, the precursor of several male reproductive tract structures. Evidence supports that testosterone is required for the maintenance of the Wolffian duct via its action on the mesenchyme. However, it remains uncertain how testosterone alone is able to facilitate formation of regionally specific structures such as the epididymis, vas deferens, and seminal vesicle from a straight Wolffian duct. In this study, we identified inhibin beta A (or\n                    <jats:italic>Inhba<\/jats:italic>\n                    ) as a regional paracrine factor in mouse mesonephroi that controls coiling of the epithelium in the anterior Wolffian duct, the future epididymis.\n                    <jats:italic>Inhba<\/jats:italic>\n                    was expressed specifically in the mesenchyme of the anterior Wolffian duct at embryonic day 12.5 before the production of androgens. In the absence of\n                    <jats:italic>Inhba<\/jats:italic>\n                    , the epididymis failed to develop the characteristic coiling in the epithelium, which showed a dramatic decrease in proliferation. This loss of epididymal coiling did not result from testosterone deficiency, because testosterone production and parameters for testosterone action such as testis descent and anogenital distance remained normal. We further found that initial\n                    <jats:italic>Inhba<\/jats:italic>\n                    expression did not require testosterone as\n                    <jats:italic>Inhba<\/jats:italic>\n                    was also expressed in the anterior Wolffian duct of female embryos where no testosterone was produced. However,\n                    <jats:italic>Inhba<\/jats:italic>\n                    expression at later stages depended on testosterone. These results demonstrated that\n                    <jats:italic>Inhba<\/jats:italic>\n                    ,\n                    <jats:italic>a<\/jats:italic>\n                    mesenchyme-specific gene, acts collectively with testosterone to facilitate epididymal coiling by stimulating epithelial proliferation.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0703445104","type":"journal-article","created":{"date-parts":[[2007,6,25]],"date-time":"2007-06-25T21:05:26Z","timestamp":1182805526000},"page":"11322-11327","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":69,"title":["Essential roles of inhibin beta A in mouse epididymal coiling"],"prefix":"10.1073","volume":"104","author":[{"given":"Jessica","family":"Tomaszewski","sequence":"first","affiliation":[{"name":"*Department of Biology, School of Integrative Biology, 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