{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T16:15:48Z","timestamp":1781108148783,"version":"3.54.1"},"reference-count":30,"publisher":"National Academy of Sciences","issue":"28","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,7,10]]},"abstract":"<jats:p>\n                    On our initial discovery that prion protein (PrP)-derived peptides were capable of capturing the pathogenic prion protein (PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    ), we have been interested in how these peptides interact with PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    . After screening peptides from the entire human PrP sequence, we found two peptides (PrP\n                    <jats:sub>19\u201330<\/jats:sub>\n                    and PrP\n                    <jats:sub>100\u2013111<\/jats:sub>\n                    ) capable of binding full-length PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    in plasma, a medium containing a complex mixture of other proteins including a vast excess of the normal prion protein (PrP\n                    <jats:sup>C<\/jats:sup>\n                    ). The limit of detection for captured PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    was calculated to be 8 amol from a \u224810\n                    <jats:sup>5<\/jats:sup>\n                    -fold dilution of 10% (wt\/vol) human variant Creutzfeldt\u2013Jakob disease brain homogenate, with &gt;3,800-fold binding specificity to PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    over PrP\n                    <jats:sup>C<\/jats:sup>\n                    . Through extensive analyses, we show that positively charged amino acids play an important, but not exclusive, role in the interaction between the peptides and PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    . Neither hydrophobic nor polar interactions appear to correlate with binding activity. The peptide\u2013PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    interaction was not sequence-specific, but amino acid composition affected binding. Binding occurs through a conformational domain that is only present in PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    , is species-independent, and is not affected by proteinase K digestion. These and other findings suggest a mechanism by which cationic domains of PrP\n                    <jats:sup>C<\/jats:sup>\n                    may play a role in the recruitment of PrP\n                    <jats:sup>C<\/jats:sup>\n                    to PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.0704260104","type":"journal-article","created":{"date-parts":[[2007,6,29]],"date-time":"2007-06-29T20:54:42Z","timestamp":1183150482000},"page":"11551-11556","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":48,"title":["Characterization of prion protein (PrP)-derived peptides that discriminate full-length PrP\n                    <sup>Sc<\/sup>\n                    from PrP\n                    <sup>C<\/sup>"],"prefix":"10.1073","volume":"104","author":[{"given":"Anthony L.","family":"Lau","sequence":"first","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice Y.","family":"Yam","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa M. D.","family":"Michelitsch","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemei","family":"Wang","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Gao","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J.","family":"Goodson","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gulliver","family":"Timoteo","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Hall","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelica","family":"Medina-Selby","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doris","family":"Coit","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Colin","family":"McCoin","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce","family":"Phelps","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Wu","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celine","family":"Hu","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Chien","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Peretz","sequence":"additional","affiliation":[{"name":"Novartis Vaccines and Diagnostics, Inc., 4560 Horton Street, Emeryville, CA 94608"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2007,7,10]]},"reference":[{"key":"e_1_3_4_1_2","volume-title":"Prion Biology and Diseases","author":"Prusiner SB","year":"2004","unstructured":"SB Prusiner Prion Biology and Diseases (Cold Spring Harbor Lab Press, 2nd Ed, Woodbury, NY, 2004).","edition":"2"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)15486-X"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16811-6"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(06)69835-8"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1997.1328"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.9413-9418.1998"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403522101"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1055"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1741-6892.2004.00517b.x"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00562a028"},{"key":"e_1_3_4_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(82)90515-0"},{"key":"e_1_3_4_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410350504"},{"key":"e_1_3_4_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63613-9"},{"key":"e_1_3_4_14_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2001.41040441.x"},{"key":"e_1_3_4_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1423-0410.2001.00112.x"},{"key":"e_1_3_4_16_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052703499"},{"key":"e_1_3_4_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402254101"},{"key":"e_1_3_4_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.26.15457"},{"key":"e_1_3_4_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/45574"},{"key":"e_1_3_4_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.3.1408-1413.2001"},{"key":"e_1_3_4_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.9.4351-4360.2000"},{"key":"e_1_3_4_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C000483200"},{"key":"e_1_3_4_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.7.3453-3461.2001"},{"key":"e_1_3_4_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.19.10069"},{"key":"e_1_3_4_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2476"},{"key":"e_1_3_4_26_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.1994.0034"},{"key":"e_1_3_4_27_2","doi-asserted-by":"publisher","DOI":"10.1067\/mlc.2001.111951"},{"key":"e_1_3_4_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79959-0"},{"key":"e_1_3_4_29_2","volume-title":"Protocols for Determination of Limits of Detection and Limits of Quantitation","author":"Tholen DW","year":"2004","unstructured":"DW Tholen, K Linnet, M Kondratovich, DA Armbruster, PE Garrett, RL Jones, MH Kroll, RM Lequin, TJ Pankratz, GA Scassellati, et al. Protocols for Determination of Limits of Detection and Limits of Quantitation (Clin and Lab Standards Inst, Wayne, PA, 2004)."},{"key":"e_1_3_4_30_2","doi-asserted-by":"publisher","DOI":"10.1086\/431602"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0704260104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T16:27:22Z","timestamp":1649780842000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0704260104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,7,10]]},"references-count":30,"journal-issue":{"issue":"28","published-print":{"date-parts":[[2007,7,10]]}},"alternative-id":["10.1073\/pnas.0704260104"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0704260104","relation":{},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,7,10]]},"assertion":[{"value":"2006-11-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-07-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}