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This study shows that a C-terminally truncated, noncleavable variant of LCMV-GP led to the accumulation of stable, soluble GP trimers in the endoplasmic reticulum (ER) of the antigen donor cell, and thereby converted LCMV-GP into a potent immunogen for cytotoxic T lymphocyte cross-priming. Immunization of mice with tumor cells expressing an ER-retained LCMV-GP variant cross-primed protective antiviral cytotoxic T lymphocyte responses\n                    <jats:italic>in vivo<\/jats:italic>\n                    at least 10,000-fold better than immunization with cells expressing the cross-presentation-\u201cresistant\u201d wild-type LCMV-GP. Thus the ER is a cellular compartment that can provide antigen for cross-presentation, and modifications affecting stability and subcellular localization of the antigen significantly increase its availability for MHC class I cross-presentation. These findings impinge on vaccine strategies.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0704423104","type":"journal-article","created":{"date-parts":[[2007,8,8]],"date-time":"2007-08-08T21:10:08Z","timestamp":1186607408000},"page":"13426-13431","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":19,"title":["A lymphocytic choriomeningitis virus glycoprotein variant that is retained in the endoplasmic reticulum efficiently cross-primes CD8\n                    <sup>+<\/sup>\n                    T cell responses"],"prefix":"10.1073","volume":"104","author":[{"given":"Stefan","family":"Freigang","sequence":"first","affiliation":[{"name":"*Institute of Experimental Immunology, Department of Pathology, University Hospital, Schmelzbergstrasse 12, CH-8091 Zurich, Switzerland;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Eschli","sequence":"additional","affiliation":[{"name":"*Institute of 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