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Here we addressed the role of Golgi actin downstream of ARF1 by using a biomimetic assay consisting of liposomes of defined lipid composition, carrying an activated form of ARF1 incubated in cytosolic cell extracts. We observed actin polymerization around the liposomes resulting in thick actin shells and actin comet tails that pushed the ARF1 liposomes forward. The assay was used to characterize the ARF1-dependent pathway, leading to actin polymerization, and confirmed a dependency on CDC42 and its downstream effector N-WASP. Overall, this study demonstrates that actin polymerization driven by the complex multicomponent signaling cascade of the Golgi apparatus can be reproduced with a biomimetic system. Moreover, our results are consistent with the view that actin-based force generation at the site of vesicle formation contributes to the mechanism of fission. In addition to its well established function in coat recruitment, the ARF1 machinery also might produce movement- and fission-promoting forces through actin polymerization.<\/jats:p>","DOI":"10.1073\/pnas.0704749104","type":"journal-article","created":{"date-parts":[[2007,10,17]],"date-time":"2007-10-17T21:25:13Z","timestamp":1192656313000},"page":"16928-16933","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":43,"title":["ARF1-mediated actin polymerization produces movement of artificial vesicles"],"prefix":"10.1073","volume":"104","author":[{"given":"Julien","family":"Heuvingh","sequence":"first","affiliation":[{"name":"*Centre de Recherche, Institut Curie, F-75248 Paris, France;"},{"name":"Unit\u00e9s Mixte de Recherche 168 and"},{"name":"144, Centre National de la Recherche Scientifique, F-75248 Paris, France;"},{"name":"Universit\u00e9 Paris VI, F-75248 Paris, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Franco","sequence":"additional","affiliation":[{"name":"Institut de Pharmacologie Mol\u00e9culaire et Cellulaire, Unit\u00e9 Mixte de Recherche 6097, Centre National de la Recherche Scientifique, F-06560 Valbonne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Chavrier","sequence":"additional","affiliation":[{"name":"*Centre de Recherche, Institut Curie, F-75248 Paris, France;"},{"name":"144, Centre National de la Recherche Scientifique, F-75248 Paris, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9cile","family":"Sykes","sequence":"additional","affiliation":[{"name":"*Centre de Recherche, Institut Curie, F-75248 Paris, France;"},{"name":"Unit\u00e9s Mixte de Recherche 168 and"},{"name":"Universit\u00e9 Paris VI, F-75248 Paris, France; 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