{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:31:12Z","timestamp":1791325872876,"version":"4.3.1"},"reference-count":27,"publisher":"National Academy of Sciences","issue":"36","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2007,9,4]]},"abstract":"\n The SaPIs are 14- to 17-kb mobile pathogenicity islands in staphylococci that carry genes for superantigen toxins and other virulence factors and are responsible for the toxic shock syndrome and other superantigen-related diseases. They reside at specific chromosomal sites and are induced by certain bacteriophages to initiate an excision-replication-packaging program, resulting in their incorporation into small infective phage-like particles. These are responsible for very high transfer frequencies that often equal and sometimes exceed the plaque-forming titer of the inducing phage. The ability of the SaPIs to replicate autonomously defines them as individual replicons and, like other prokaryotic replicons, they possess replicon-specific initiation functions. In this paper, we report identification of the SaPI replication origin (\n ori<\/jats:italic>\n ) and replication initiation protein (Rep), which has helicase as well as initiation activity. The SaPI\n oris<\/jats:italic>\n are binding sites for the respective Rep proteins and consist of multiple oligonucleotide repeats in two sets, flanking an AT-rich region that may be the site of initial melting. Plasmids containing the\n rep-ori<\/jats:italic>\n complex plus an additional gene,\n pri<\/jats:italic>\n , can replicate autonomously in\n Staphylococcus aureus<\/jats:italic>\n but are very unstable, probably because of defective segregation.\n <\/jats:p>","DOI":"10.1073\/pnas.0705994104","type":"journal-article","created":{"date-parts":[[2007,8,10]],"date-time":"2007-08-10T21:01:03Z","timestamp":1186779663000},"page":"14182-14188","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":65,"title":["A pathogenicity island replicon in\n Staphylococcus aureus<\/i>\n replicates as an unstable plasmid"],"prefix":"10.1073","volume":"104","author":[{"given":"Carles","family":"\u00dabeda","sequence":"first","affiliation":[{"name":"*Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, Departments of Microbiology and Medicine, New York, NY 10016; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Barry","sequence":"additional","affiliation":[{"name":"*Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, Departments of Microbiology and Medicine, New York, NY 10016; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 R.","family":"Penad\u00e9s","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y Tecnolog\u00eda Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), Apdo. 187, 12.400 Segorbe, Castell\u00f3n, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard P.","family":"Novick","sequence":"additional","affiliation":[{"name":"*Kimmel Center for Biology and Medicine of the Skirball Institute, New York University School of Medicine, Departments of Microbiology and Medicine, New York, NY 10016; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2007,9,4]]},"reference":[{"key":"e_1_3_4_1_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00947.x"},{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.1.63-70.2001"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03577.x"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2001.02488.x"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1159\/000100857"},{"key":"e_1_3_4_6_2","author":"Maiques E","year":"2007","unstructured":"E Maiques, C \u00dabeda, MA Tormo, MD Ferrer, I Lasa, RP Novick, JR Penad\u00e9s J Bacteriol, in press. 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