{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T10:36:25Z","timestamp":1790937385964,"version":"4.1.0"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"15","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,4,15]]},"abstract":"<jats:p>\n                    The proprotein convertase PC5\/6 cleaves protein precursors after basic amino acids and is essential for implantation in CD1\/129\/Sv\/C57BL\/6 mixed-background mice. Conditional inactivation of\n                    <jats:italic>Pcsk5<\/jats:italic>\n                    in the epiblast but not in the extraembryonic tissue bypassed early embryonic lethality but resulted in death at birth. PC5\/6-deficient embryos exhibited Gdf11-related phenotypes such as altered anteroposterior patterning with extra vertebrae and lack of tail and kidney agenesis. They also exhibited Gdf11-independent phenotypes, such as a smaller size, multiple hemorrhages, collapsed alveoli, and retarded ossification.\n                    <jats:italic>In situ<\/jats:italic>\n                    hybridization revealed overlapping PC5\/6 and Gdf11 mRNA expression patterns.\n                    <jats:italic>In vitro<\/jats:italic>\n                    and\n                    <jats:italic>ex vivo<\/jats:italic>\n                    analyses showed that the selectivity of PC5\/6 for Gdf11 essentially resides in the presence of a P1\u2032 Asn in the RSRR\u2193N cleavage motif. This work identifies Gdf11 as a likely\n                    <jats:italic>in vivo<\/jats:italic>\n                    specific substrate of PC5\/6 and opens the way to the identification of other key substrates of this convertase.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0709428105","type":"journal-article","created":{"date-parts":[[2008,3,31]],"date-time":"2008-03-31T21:09:39Z","timestamp":1206997779000},"page":"5750-5755","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":103,"title":["<i>In vivo<\/i>\n                    functions of the proprotein convertase PC5\/6 during mouse development: Gdf11 is a likely substrate"],"prefix":"10.1073","volume":"105","author":[{"given":"Rachid","family":"Essalmani","sequence":"first","affiliation":[{"name":"Laboratory of Biochemical Neuroendocrinology, Clinical Research Institute of Montreal, 110 Pine Avenue West, Montreal, QC, Canada H2W 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