{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:13:43Z","timestamp":1791350023110,"version":"4.3.1"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"10","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,3,11]]},"abstract":"<jats:p>\n                    During morphogenesis, the actin cytoskeleton mediates cell-shape change in response to growth signals. In plants, actin filaments organize the cytoplasm in regions of polarized growth, and the filamentous arrays can be highly dynamic. Small GTPase signaling proteins termed Rho of plants (ROP)\/RAC control actin polymerization. ROPs cycle between inactive GDP-bound and active GTP-bound forms, and it is the active form that interacts with effector proteins to mediate cytoskeletal rearrangement and cell-shape change. A class of proteins termed guanine nucleotide exchange factors (GEFs) generate GTP\u2013ROP and positively regulate ROP signaling. However, in almost all experimental systems, it has proven difficult to unravel the complex signaling pathways from GEFs to the proteins that nucleate actin filaments. In this article, we show that the DOCK family protein SPIKE1 (SPK1) is a GEF, and that one function of SPK1 is to control actin polymerization via two heteromeric complexes termed WAVE and actin-related protein (ARP) 2\/3. The genetic pathway was constructed by using a combination of highly informative\n                    <jats:italic toggle=\"yes\">spk1<\/jats:italic>\n                    alleles and detailed analyses of\n                    <jats:italic toggle=\"yes\">spk1<\/jats:italic>\n                    ,\n                    <jats:italic toggle=\"yes\">wave<\/jats:italic>\n                    , and\n                    <jats:italic toggle=\"yes\">arp2<\/jats:italic>\n                    \/\n                    <jats:italic toggle=\"yes\">3<\/jats:italic>\n                    single and double mutants. Remarkably, we find that in addition to providing GEF activity, SPK1 associates with WAVE complex proteins and may spatially organize signaling. Our results describe a unique regulatory scheme for ARP2\/3 regulation in cells, one that can be tested for widespread use in other multicellular organisms.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0710294105","type":"journal-article","created":{"date-parts":[[2008,2,28]],"date-time":"2008-02-28T21:11:37Z","timestamp":1204233097000},"page":"4044-4049","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":144,"title":["A SPIKE1 signaling complex controls actin-dependent cell morphogenesis through the heteromeric WAVE and ARP2\/3 complexes"],"prefix":"10.1073","volume":"105","author":[{"given":"Dipanwita","family":"Basu","sequence":"first","affiliation":[{"name":"Department of Agronomy, Purdue University, Lilly Hall, 915 W. 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