{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T10:58:27Z","timestamp":1791284307963,"version":"4.1.0"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"15","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,4,15]]},"abstract":"<jats:p>\n                    The phenolic methyl ether 3,5-dimethoxytoluene (DMT) is a major scent compound of many modern rose varieties, and its fragrance participates in the characteristic \u201ctea scent\u201d that gave their name to Tea and Hybrid Tea roses. Among wild roses, phenolic methyl ether (PME) biosynthesis is restricted to Chinese rose species, but the progenitors of modern roses included both European and Chinese species (e.g.,\n                    <jats:italic>Rosa chinensis<\/jats:italic>\n                    cv Old Blush), so this trait was transmitted to their hybrid progeny. The last steps of the biosynthetic pathways leading to DMT involve two methylation reactions catalyzed by the highly similar orcinol\n                    <jats:italic>O<\/jats:italic>\n                    -methyltransferases (OOMT) 1 and 2. OOMT1 and OOMT2 enzymes exhibit different substrate specificities that are consistent with their operating sequentially in DMT biosynthesis. Here, we show that these different substrate specificities are mostly due to a single amino acid polymorphism in the phenolic substrate binding site of OOMTs. An analysis of the\n                    <jats:italic>OOMT<\/jats:italic>\n                    gene family in 18 species representing the diversity of the genus\n                    <jats:italic>Rosa<\/jats:italic>\n                    indicated that only Chinese roses possess both the\n                    <jats:italic>OOMT2<\/jats:italic>\n                    and the\n                    <jats:italic>OOMT1<\/jats:italic>\n                    genes. In addition, we provide evidence that the Chinese-rose-specific\n                    <jats:italic>OOMT1<\/jats:italic>\n                    genes most probably evolved from an\n                    <jats:italic>OOMT2<\/jats:italic>\n                    -like gene that has homologues in the genomes of all extant roses. We propose that the emergence of the\n                    <jats:italic>OOMT1<\/jats:italic>\n                    gene may have been a critical step in the evolution of scent production in Chinese roses.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0711551105","type":"journal-article","created":{"date-parts":[[2008,4,14]],"date-time":"2008-04-14T21:10:43Z","timestamp":1208207443000},"page":"5927-5932","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":108,"title":["Scent evolution in Chinese roses"],"prefix":"10.1073","volume":"105","author":[{"given":"Gabriel","family":"Scalliet","sequence":"first","affiliation":[{"name":"Laboratoire Reproduction et D\u00e9veloppement des Plantes, Institut F\u00e9d\u00e9ratif de Recherche 128, Unit\u00e9 Mixte de Recherche 5667, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universit\u00e9 Lyon 1, Ecole Normale Sup\u00e9rieure de Lyon, 46 All\u00e9e d'Italie, 69364 Lyon Cedex 07, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florence","family":"Piola","sequence":"additional","affiliation":[{"name":"Laboratoire d'Ecologie des Hydrosyst\u00e8mes Fluviaux, Unit\u00e9 Mixte de Recherche 5023, Centre National de la Recherche Scientifique, Universit\u00e9 Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christophe J.","family":"Douady","sequence":"additional","affiliation":[{"name":"Laboratoire d'Ecologie des Hydrosyst\u00e8mes Fluviaux, Unit\u00e9 Mixte de Recherche 5023, Centre National de la Recherche Scientifique, Universit\u00e9 Lyon 1, 43 Boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"R\u00e9ty","sequence":"additional","affiliation":[{"name":"Laboratoire de Cristallographie et RMN Biologique, Unit\u00e9 Mixte de Recherche 8015, Centre National de la Recherche Scientifique-Facult\u00e9 de Pharmacie, 4 Avenue de l'Observatoire, case 48, 75270 Paris Cedex 06, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Raymond","sequence":"additional","affiliation":[{"name":"Laboratoire Reproduction et D\u00e9veloppement des Plantes, Institut F\u00e9d\u00e9ratif de Recherche 128, Unit\u00e9 Mixte de Recherche 5667, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universit\u00e9 Lyon 1, Ecole Normale Sup\u00e9rieure de Lyon, 46 All\u00e9e d'Italie, 69364 Lyon Cedex 07, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Baudino","sequence":"additional","affiliation":[{"name":"Laboratoire de Biotechnologies V\u00e9g\u00e9tales Appliqu\u00e9es aux Plantes Aromatiques et M\u00e9dicinales, EA3061, Universit\u00e9 Jean Monnet, 23 Rue du Dr. Michelon, 42023 Saint-Etienne Cedex 2, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karim","family":"Bordji","sequence":"additional","affiliation":[{"name":"Laboratoire Reproduction et D\u00e9veloppement des Plantes, Institut F\u00e9d\u00e9ratif de Recherche 128, Unit\u00e9 Mixte de Recherche 5667, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universit\u00e9 Lyon 1, Ecole Normale Sup\u00e9rieure de Lyon, 46 All\u00e9e d'Italie, 69364 Lyon Cedex 07, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammed","family":"Bendahmane","sequence":"additional","affiliation":[{"name":"Laboratoire Reproduction et D\u00e9veloppement des Plantes, Institut F\u00e9d\u00e9ratif de Recherche 128, Unit\u00e9 Mixte de Recherche 5667, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universit\u00e9 Lyon 1, Ecole Normale Sup\u00e9rieure de Lyon, 46 All\u00e9e d'Italie, 69364 Lyon Cedex 07, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Dumas","sequence":"additional","affiliation":[{"name":"Laboratoire Reproduction et D\u00e9veloppement des Plantes, Institut F\u00e9d\u00e9ratif de Recherche 128, Unit\u00e9 Mixte de Recherche 5667, Centre National de la Recherche Scientifique, Institut National de la Recherche Agronomique, Universit\u00e9 Lyon 1, Ecole Normale Sup\u00e9rieure de Lyon, 46 All\u00e9e d'Italie, 69364 Lyon Cedex 07, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. 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