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Most remarkably, classification of the genes by their expression profiles revealed 35 genes exhibiting immediate-early expression. Although a similar kinetic class has been described for other virus families, to our knowledge, this is the first demonstration of its existence in orthopoxviruses. Despite expression levels higher than for genes in the other three kinetic classes, the functions of more than half of these remain unknown. Additionally, genes within each kinetic class were spatially grouped together in the genome. This genome-wide picture of transcription alters our understanding of how orthopoxviruses regulate gene expression.<\/jats:p>","DOI":"10.1073\/pnas.0711573105","type":"journal-article","created":{"date-parts":[[2008,2,1]],"date-time":"2008-02-01T21:07:55Z","timestamp":1201900075000},"page":"2140-2145","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":172,"title":["Kinetic analysis of a complete poxvirus transcriptome reveals an immediate-early class of genes"],"prefix":"10.1073","volume":"105","author":[{"given":"Erika","family":"Assarsson","sequence":"first","affiliation":[{"name":"*Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason A.","family":"Greenbaum","sequence":"additional","affiliation":[{"name":"*Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Sundstr\u00f6m","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lana","family":"Schaffer","sequence":"additional","affiliation":[{"name":"DNA Array Core Facility, The Scripps Research Institute, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer A.","family":"Hammond","sequence":"additional","affiliation":[{"name":"DNA Array Core Facility, The Scripps Research Institute, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Pasquetto","sequence":"additional","affiliation":[{"name":"*Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Oseroff","sequence":"additional","affiliation":[{"name":"*Division of Vaccine Discovery, La Jolla Institute for Allergy and Immunology, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. 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