{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T20:42:51Z","timestamp":1792701771403,"version":"4.5.0"},"reference-count":39,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,4,8]]},"abstract":"<jats:p>\n                    Human species C adenovirus serotype 5 (Ad5) is the most common viral vector used in clinical studies worldwide. Ad5 vectors infect liver cells\n                    <jats:italic>in vivo<\/jats:italic>\n                    with high efficiency via a poorly defined mechanism, which involves virus binding to vitamin K-dependent blood coagulation factors. Here, we report that the major Ad5 capsid protein, hexon, binds human coagulation factor X (FX) with an affinity of 229 pM. This affinity is 40-fold stronger than the reported affinity of Ad5 fiber for the cellular receptor coxsackievirus and adenovirus receptor, CAR. Cryoelectron microscopy and single-particle image reconstruction revealed that the FX attachment site is localized to the central depression at the top of the hexon trimer. Hexon-mutated virus bearing a large insertion in hexon showed markedly reduced FX binding\n                    <jats:italic>in vitro<\/jats:italic>\n                    and failed to deliver a transgene to hepatocytes\n                    <jats:italic>in vivo<\/jats:italic>\n                    . This study describes the mechanism of FX binding to Ad5 and demonstrates the critical role of hexon for virus infection of hepatocytes\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1073\/pnas.0711757105","type":"journal-article","created":{"date-parts":[[2008,4,7]],"date-time":"2008-04-07T21:06:33Z","timestamp":1207602393000},"page":"5483-5488","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":314,"title":["Adenovirus serotype 5 hexon is critical for virus infection of hepatocytes\n                    <i>in vivo<\/i>"],"prefix":"10.1073","volume":"105","author":[{"given":"O.","family":"Kalyuzhniy","sequence":"first","affiliation":[{"name":"*Division of Medical Genetics, Department of Medicine, University of Washington, Seattle, WA 98195-7720;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N. 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