{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T23:20:47Z","timestamp":1792452047588,"version":"4.5.0"},"reference-count":52,"publisher":"National Academy of Sciences","issue":"25","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,6,24]]},"abstract":"<jats:p>The aggregation of proteins into amyloid fibrils is associated with several neurodegenerative diseases. In Parkinson's disease it is believed that the aggregation of \u03b1-synuclein (\u03b1-syn) from monomers by intermediates into amyloid fibrils is the toxic disease-causative mechanism. Here, we studied the structure of \u03b1-syn in its amyloid state by using various biophysical approaches. Quenched hydrogen\/deuterium exchange NMR spectroscopy identified five \u03b2-strands within the fibril core comprising residues 35\u201396 and solid-state NMR data from amyloid fibrils comprising the fibril core residues 30\u2013110 confirmed the presence of \u03b2-sheet secondary structure. The data suggest that \u03b21-strand interacts with \u03b22, \u03b22 with \u03b23, \u03b23 with \u03b24, and \u03b24 with \u03b25. High-resolution cryoelectron microscopy revealed the protofilament boundaries of \u22482 \u00d7 3.5 nm. Based on the combination of these data and published structural studies, a fold of \u03b1-syn in the fibrils is proposed and discussed.<\/jats:p>","DOI":"10.1073\/pnas.0712179105","type":"journal-article","created":{"date-parts":[[2008,6,12]],"date-time":"2008-06-12T21:34:14Z","timestamp":1213306454000},"page":"8637-8642","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":541,"title":["The fold of \u03b1-synuclein fibrils"],"prefix":"10.1073","volume":"105","author":[{"given":"Mar\u00e7al","family":"Vilar","sequence":"first","affiliation":[{"name":"The Salk Institute for Biological Studies, North Torrey Pines Road, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Ting","family":"Chou","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology, University of California, 1 Shields Avenue, Davis, CA 95616; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thorsten","family":"L\u00fchrs","sequence":"additional","affiliation":[{"name":"The Salk Institute for Biological Studies, North Torrey Pines Road, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samir K.","family":"Maji","sequence":"additional","affiliation":[{"name":"The Salk Institute for Biological Studies, North Torrey Pines Road, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique","family":"Riek-Loher","sequence":"additional","affiliation":[{"name":"The Salk Institute for Biological Studies, North Torrey Pines Road, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene","family":"Verel","sequence":"additional","affiliation":[{"name":"Laboratory of Physical Chemistry, Eidgen\u00f6ssische Technische Hochschule Zurich, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Manning","sequence":"additional","affiliation":[{"name":"Laboratory of Physical Chemistry, Eidgen\u00f6ssische Technische Hochschule Zurich, 8093 Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Stahlberg","sequence":"additional","affiliation":[{"name":"Molecular and Cellular Biology, University of California, 1 Shields Avenue, Davis, CA 95616; 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