{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:05:23Z","timestamp":1791223523103,"version":"4.1.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"23","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,6,10]]},"abstract":"<jats:p>\n                    The human T cell leukemia virus type 1 (HTLV-1) is the causative agent of adult T cell leukemia\/lymphoma. The multifunctional virally encoded oncoprotein Tax is responsible for malignant transformation and potent activation of HTLV-1 transcription. Tax, in complex with phosphorylated cAMP response element binding protein (pCREB), strongly recruits the cellular coactivators CREB binding protein (CBP)\/p300 to the viral promoter concomitant with transcriptional activation. Although the mechanism of activator\/coactivator-mediated transcriptional activation is poorly understood, the recruitment of CBP\/p300 by regulatory factors appears to function, in part, by promoting changes in chromatin architecture that are permissive to transcriptional activation. Here, we show that CBP\/p300 recruitment promotes histone acetylation and eviction of the histone octamer from the chromatin-assembled HTLV-1 promoter template\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Nucleosome disassembly is strictly acetyl-CoA dependent and is not linked to ATP utilization. We find that the histone chaperone, nucleosome assembly protein 1 (NAP1), cooperates with CBP\/p300 in eviction of the acetylated histones from the chromatin template. These findings reveal a unique mechanism in which the DNA-bound Tax\/pCREB complex recruits CBP\/p300, and together with NAP1, the coactivators cooperate to dramatically reduce nucleosome occupancy at the viral promoter in an acetylation-dependent and transcription-independent fashion.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0800534105","type":"journal-article","created":{"date-parts":[[2008,6,3]],"date-time":"2008-06-03T22:48:50Z","timestamp":1212533330000},"page":"7959-7963","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":71,"title":["The coactivators CBP\/p300 and the histone chaperone NAP1 promote transcription-independent nucleosome eviction at the HTLV-1 promoter"],"prefix":"10.1073","volume":"105","author":[{"given":"Neelam","family":"Sharma","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Campus Box 1870, Colorado State University, Fort Collins, CO 80523"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer K.","family":"Nyborg","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology, Campus Box 1870, Colorado State University, Fort Collins, CO 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