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Upon red light illumination, the fusion proteins bind each other, activating WASP, and consequently stimulating actin assembly by the WASP target, the Arp2\/3 complex. Binding and WASP activation are reversed by far-red illumination. Our approach, in which the biochemical specificity of the nucleotide switch in Cdc42 is overridden by the light-dependent PhyB-Pif3 interaction, should be generally applicable to other GTPase-effector pairs.<\/jats:p>","DOI":"10.1073\/pnas.0801232105","type":"journal-article","created":{"date-parts":[[2008,8,26]],"date-time":"2008-08-26T20:45:01Z","timestamp":1219783501000},"page":"12797-12802","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":94,"title":["Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2\/3 complex pathway"],"prefix":"10.1073","volume":"105","author":[{"given":"Daisy W.","family":"Leung","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute and"},{"name":"Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chinatsu","family":"Otomo","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanne","family":"Chory","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and Plant Biology Laboratory, The Salk Institute, La Jolla, CA 92037"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael K.","family":"Rosen","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute and"},{"name":"Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas, TX 75390; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2008,9,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-007-1131-5"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2005.10.010"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2005.07.003"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.55.030193.003543"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200500531"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth936"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.38.072902.092259"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm728"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.56.032604.144208"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.025643"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81636-0"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/23500"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.104.022350"},{"key":"e_1_3_3_14_2","first-page":"2271","article-title":"Biochemical characterization of Arabidopsis wild-type and mutant phytochrome B holoproteins","volume":"9","author":"Elich TD","year":"1997","unstructured":"TD Elich, J Chory, Biochemical characterization of Arabidopsis wild-type and mutant phytochrome B holoproteins. 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