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Here we report the x-ray structure of HsAQP5 to 2.0-\u212b resolution and highlight structural similarities and differences relative to other eukaryotic aquaporins. A lipid occludes the putative central pore, preventing the passage of gas or ions through the center of the tetramer. Multiple consensus phosphorylation sites are observed in the structure and their potential regulatory role is discussed. We postulate that a change in the conformation of the C terminus may arise from the phosphorylation of AQP5 and thereby signal trafficking.<\/jats:p>","DOI":"10.1073\/pnas.0801466105","type":"journal-article","created":{"date-parts":[[2008,9,3]],"date-time":"2008-09-03T20:53:59Z","timestamp":1220475239000},"page":"13327-13332","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":201,"title":["High-resolution x-ray structure of human aquaporin 5"],"prefix":"10.1073","volume":"105","author":[{"given":"Rob","family":"Horsefield","sequence":"first","affiliation":[{"name":"*Department of Chemistry, Biochemistry and Biophysics, University of Gothenburg, Box 462, SE-405 30 G\u00f6teborg, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristina","family":"Nord\u00e9n","sequence":"additional","affiliation":[{"name":"Center for Molecular Protein Science, Department of Biochemistry, Lund University, Box 124, SE-221 00 Lund, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Fellert","sequence":"additional","affiliation":[{"name":"Center for Molecular Protein Science, Department of Biochemistry, Lund University, Box 124, SE-221 00 Lund, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Backmark","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Bioscience, Chalmers University of Technology, Box 462, SE-405 30 G\u00f6teborg, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanna","family":"T\u00f6rnroth-Horsefield","sequence":"additional","affiliation":[{"name":"*Department of Chemistry, Biochemistry and Biophysics, University of Gothenburg, Box 462, SE-405 30 G\u00f6teborg, Sweden;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anke C.","family":"Terwisscha van Scheltinga","sequence":"additional","affiliation":[{"name":"Department of Structural Biology, 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