{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:03:57Z","timestamp":1792569837448,"version":"4.5.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"38","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,9,23]]},"abstract":"<jats:p>\n                    The spatiotemporal properties of the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -release process in skeletal muscle fibers from normal and\n                    <jats:italic>mdx<\/jats:italic>\n                    fibers were determined using the confocal-spot detection technique. The Ca\n                    <jats:sup>2+<\/jats:sup>\n                    indicator OGB-5N was used to record action potential-evoked fluorescence signals at consecutive locations separated by 200 nm along multiple sarcomeres of\n                    <jats:italic>FDB<\/jats:italic>\n                    fibers loaded with 10- and 30-mM EGTA. Three-dimensional reconstructions of fluorescence transients demonstrated the existence of microdomains of increased fluorescence around the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -release sites in both mouse strains. The Ca\n                    <jats:sup>2+<\/jats:sup>\n                    microdomains in\n                    <jats:italic>mdx<\/jats:italic>\n                    fibers were regularly spaced along the fiber axis, displaying a distribution similar to that seen in normal fibers. Nevertheless, both preparations differed in that in 10-mM EGTA Ca\n                    <jats:sup>2+<\/jats:sup>\n                    microdomains had smaller amplitudes and were wider in\n                    <jats:italic>mdx<\/jats:italic>\n                    fibers than in controls. In addition, Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -dependent fluorescence transients recorded at selected locations within the sarcomere of\n                    <jats:italic>mdx<\/jats:italic>\n                    muscle fibers were not only smaller, but also slower than their counterparts in normal fibers. Notably, differences in the spatial features of the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    microdomains recorded in\n                    <jats:italic>mdx<\/jats:italic>\n                    and normal fibers, but not in the amplitude and kinetics of the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    transients, were eliminated in 30-mM EGTA. Our results consistently demonstrate that Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -release flux calculated from release sites in\n                    <jats:italic>mdx<\/jats:italic>\n                    fibers is uniformly impaired with respect to those normal fibers. The Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -release reduction is consistent with that previously measured using global detection techniques.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0802217105","type":"journal-article","created":{"date-parts":[[2008,9,11]],"date-time":"2008-09-11T21:10:06Z","timestamp":1221167406000},"page":"14698-14703","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":35,"title":["Dystrophic skeletal muscle fibers display alterations at the level of calcium microdomains"],"prefix":"10.1073","volume":"105","author":[{"given":"Marino","family":"DiFranco","sequence":"first","affiliation":[{"name":"Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher E.","family":"Woods","sequence":"additional","affiliation":[{"name":"Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joana","family":"Capote","sequence":"additional","affiliation":[{"name":"Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio L.","family":"Vergara","sequence":"additional","affiliation":[{"name":"Department of Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90095"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2008,9,23]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90579-4"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07815-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005545325254"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1152\/japplphysiol.01242.2001"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.2001.00591.x"},{"key":"e_1_3_3_6_2","first-page":"257","article-title":"The functional status of dystrophic muscles and functional recovery by skeletal muscles following myoblast transfer","volume":"7","author":"Faulkner JA","year":"1997","unstructured":"JA Faulkner, SV Brooks, JR Dennis, GS Lynch, The functional status of dystrophic muscles and functional recovery by skeletal muscles following myoblast transfer. 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