{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:09:39Z","timestamp":1781104179103,"version":"3.54.1"},"reference-count":35,"publisher":"National Academy of Sciences","issue":"21","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,5,27]]},"abstract":"<jats:p>\n                    Two minichromosomes (\u03b1 and \u03b4) in addition to two other aberrant chromosomes (\u03b2 and \u03b3) were found in a transgenic\n                    <jats:italic>Arabidopsis<\/jats:italic>\n                    plant produced by an\n                    <jats:italic>in planta<\/jats:italic>\n                    vacuum infiltration technique. The minichromosomes were successfully separated by successive crossing and selfing and added to wild-type Columbia (Col-0) as a supernumerary chromosome. FISH indicated that both of the two minichromosomes originated from the short arm of chromosome 2. The mini \u03b1 chromosome contained the whole short-arm 2S and a truncated centromere (180-bp repeat cluster), whereas mini \u03b4 lacked the terminal region including telomere repeats. Pachytene FISH clearly revealed that mini \u03b4 comprised a ring chromosome carrying two copies of the region from the 180-bp repeat cluster to BAC-F3C11. Both of the 180-bp clusters (each \u2248500 kb in length) were thought to possess normal centromere functions because the centromere-specific histone H3 variant (HTR12) was detected on both clusters. Notwithstanding this dicentric and ring form, mini \u03b4 was stably transmitted to the next generations, perhaps because of its compact size (&lt;4 Mb). Chromosome \u03b2 also comprised a dicentric-like structure, with one of the two 180-bp repeat sites derived from chromosome 1 and the other from chromosome 2. However, the latter was quite small and failed to bind HTR12. The data obtained in this study indicated that 500 kb of the 180-bp array of the chromosome 2 centromere, from the edge of the 180-bp array on the short-arm side, is sufficient to form a functional domain.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0802828105","type":"journal-article","created":{"date-parts":[[2008,5,21]],"date-time":"2008-05-21T21:30:13Z","timestamp":1211405413000},"page":"7511-7516","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":21,"title":["Functional analysis of the\n                    <i>Arabidopsis<\/i>\n                    centromere by T-DNA insertion-induced centromere breakage"],"prefix":"10.1073","volume":"105","author":[{"given":"Minoru","family":"Murata","sequence":"first","affiliation":[{"name":"*Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etsuko","family":"Yokota","sequence":"additional","affiliation":[{"name":"*Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fukashi","family":"Shibata","sequence":"additional","affiliation":[{"name":"*Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan; and"},{"name":"Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazunari","family":"Kashihara","sequence":"additional","affiliation":[{"name":"*Research Institute for Bioresources, Okayama University, Kurashiki 710-0046, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2008,5,27]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1093\/oso\/9780198577812.001.0001","volume-title":"The Centromere","author":"Choo KHA","year":"1997","unstructured":"KHA Choo The Centromere (Oxford Univ Press, Oxford), pp. 97\u2013142 (1997)."},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.2174\/1389202023350228"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1266\/jjg.69.361"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5449.2468"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/35048692"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/9.4.117"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/163.3.1221"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.01144"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1266\/ggs.79.139"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10577-005-1016-3"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.681703"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kve018"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1159\/000082395"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00412-005-0045-1"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1997.12010031.x"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.12.3351"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erg034"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.8316832"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5266(98)80014-9"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010425"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:CHRO.0000036585.44138.4b"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/23.4.315"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1104\/pp.123.3.895"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/45471"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1159\/000056981"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091110398"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/4.12.2189"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004120050240"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509650103"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504235102"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anngen.2003.10.005"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200305065"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.0030179"},{"key":"e_1_3_3_34_2","first-page":"1194","article-title":"In planta Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis thaliana plants","volume":"316","author":"Bechtold N","year":"1993","unstructured":"N Bechtold, J Ellis, G Pelletier, In planta Agrobacterium-mediated gene transfer by infiltration of adult Arabidopsis thaliana plants. 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