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We found 15% of IgG memory B cell antibodies highly reactive and specific for SLE-associated extractable nuclear antigens (ENA) Ro52 and La in one patient with serum autoantibody titers of the same specificity but not in the other three patients or healthy individuals. The germ-line forms of the ENA antibodies were non-self-reactive or polyreactive with low binding to Ro52, supporting the idea that somatic mutations contributed to autoantibody specificity and reactivity. Heterogeneity in the frequency of memory B cells expressing SLE-associated autoantibodies suggests that this variable may be important in the outcome of therapies that ablate this compartment.<\/jats:p>","DOI":"10.1073\/pnas.0803644105","type":"journal-article","created":{"date-parts":[[2008,7,9]],"date-time":"2008-07-09T21:09:33Z","timestamp":1215637773000},"page":"9727-9732","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":219,"title":["Autoreactive IgG memory antibodies in patients with systemic lupus erythematosus arise from nonreactive and polyreactive precursors"],"prefix":"10.1073","volume":"105","author":[{"given":"Brun","family":"Mietzner","sequence":"first","affiliation":[{"name":"*Max Planck Institute for Infection Biology, 10117 Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Tsuiji","sequence":"additional","affiliation":[{"name":"The Rockefeller University, New York, NY 10021;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Scheid","sequence":"additional","affiliation":[{"name":"The Rockefeller University, New York, NY 10021;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klara","family":"Velinzon","sequence":"additional","affiliation":[{"name":"The Rockefeller University, New York, NY 10021;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Tiller","sequence":"additional","affiliation":[{"name":"*Max Planck Institute for Infection Biology, 10117 Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus","family":"Abraham","sequence":"additional","affiliation":[{"name":"Central Institute for Laboratory Medicine and Pathobiochemistry, Charit\u00e9 Hospital, 13353 Berlin, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose B.","family":"Gonzalez","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry and Pathobiochemistry, Charit\u00e9 Hospital, 12200 Berlin, Germany; 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