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The other is the endogenous \u03b2-myosin heavy chain (bMHC) gene modified to code for a YFP\u2013bMHC fusion protein. Using high-resolution confocal microscopy, we determined the expression of the two indicator genes in individual cardiomyocytes perinatally and after inducing cardiac hypertrophy by transverse aortic constriction. Our results provide strong evidence that the cardiac genes respond by switching their expression in an on\/off rather than graded manner, and that responding genes within a single cell and within the two nuclei of cardiomyocytes do not necessarily switch concordantly.<\/jats:p>","DOI":"10.1073\/pnas.0805120105","type":"journal-article","created":{"date-parts":[[2008,8,28]],"date-time":"2008-08-28T21:10:38Z","timestamp":1219957838000},"page":"13063-13068","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":20,"title":["Discordant on\/off switching of gene expression in myocytes during cardiac hypertrophy\n                    <i>in vivo<\/i>"],"prefix":"10.1073","volume":"105","author":[{"given":"Kumar","family":"Pandya","sequence":"first","affiliation":[{"name":"Departments of *Pathology and Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Cowhig","sequence":"additional","affiliation":[{"name":"Departments of *Pathology and Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joe","family":"Brackhan","sequence":"additional","affiliation":[{"name":"Departments of *Pathology and Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyung Suk","family":"Kim","sequence":"additional","affiliation":[{"name":"Departments of *Pathology and Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Hagaman","sequence":"additional","affiliation":[{"name":"Departments of *Pathology and Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mauricio","family":"Rojas","sequence":"additional","affiliation":[{"name":"Departments of *Pathology and Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Charles W.","family":"Carter","sequence":"additional","affiliation":[{"name":"Biochemistry and Biophysics, University of North Carolina, Chapel Hill, NC 27599; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Mao","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University, Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Howard A.","family":"Rockman","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University, Durham, NC 27710"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyo","family":"Maeda","sequence":"additional","affiliation":[{"name":"Departments of *Pathology and Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Smithies","sequence":"additional","affiliation":[{"name":"Departments of *Pathology and Laboratory Medicine and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2008,9,2]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1071545"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.026"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.09.013"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.103004.142710"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.007"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.40.110405.090555"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.02.005"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.01.015"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ento.51.110104.151007"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.75.103004.142422"},{"key":"e_1_3_3_11_2","first-page":"342","volume-title":"Molecular and Cellular Biology","author":"Lodish H","year":"2000","unstructured":"H Lodish, A Berk, S Zipursky, P Matsudaira, D Baltimore, J Darnell, Regulation of transcription initiation. 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