{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:08:46Z","timestamp":1792674526536,"version":"4.5.0"},"reference-count":25,"publisher":"National Academy of Sciences","issue":"6","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,2,10]]},"abstract":"\n Mincle (also called as Clec4e and Clecsf9) is a C-type lectin receptor expressed in activated phagocytes. Recently, we have demonstrated that Mincle is an FcR\u03b3-associated activating receptor that senses damaged cells. To search an exogenous ligand(s), we screened pathogenic fungi using cell line expressing Mincle, FcR\u03b3, and NFAT-GFP reporter. We found that Mincle specifically recognizes the\n Malassezia<\/jats:italic>\n species among 50 different fungal species tested.\n Malassezia<\/jats:italic>\n is a pathogenic fungus that causes skin diseases, such as tinea versicolor and atopic dermatitis, and fatal sepsis. However, the specific receptor on host cells has not been identified. Mutation of the putative mannose-binding motif within C-type lectin domain of Mincle abrogated\n Malassezia<\/jats:italic>\n recognition. Analyses of glycoconjugate microarray revealed that Mincle selectively binds to \u03b1-mannose but not mannan. Thus, Mincle may recognize specific geometry of \u03b1-mannosyl residues on\n Malassezia<\/jats:italic>\n species and use this to distinguish them from other fungi.\n Malassezia<\/jats:italic>\n activated macrophages to produce inflammatory cytokines\/chemokines. To elucidate the physiological function of Mincle, Mincle-deficient mice were established.\n Malassezia<\/jats:italic>\n -induced cytokine\/chemokine production by macrophages from Mincle\n \u2212\/\u2212<\/jats:sup>\n mice was significantly impaired. In vivo inflammatory responses against\n Malassezia<\/jats:italic>\n was also impaired in Mincle\n \u2212\/\u2212<\/jats:sup>\n mice. These results indicate that Mincle is the first specific receptor for\n Malassezia<\/jats:italic>\n species to be reported and plays a crucial role in immune responses to this fungus.\n <\/jats:p>","DOI":"10.1073\/pnas.0805177106","type":"journal-article","created":{"date-parts":[[2009,1,26]],"date-time":"2009-01-26T20:45:51Z","timestamp":1233002751000},"page":"1897-1902","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":391,"title":["C-type lectin Mincle is an activating receptor for pathogenic fungus,\n Malassezia<\/i>"],"prefix":"10.1073","volume":"106","author":[{"given":"Sho","family":"Yamasaki","sequence":"first","affiliation":[{"name":"Laboratory for Cell Signaling, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Makoto","family":"Matsumoto","sequence":"additional","affiliation":[{"name":"Department of Immunology and Medical Zoology, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Osamu","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan;"},{"name":"Laboratory of Host Defence,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuhiro","family":"Matsuzawa","sequence":"additional","affiliation":[{"name":"Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eri","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Laboratory for Cell Signaling, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Machie","family":"Sakuma","sequence":"additional","affiliation":[{"name":"Laboratory for Cell Signaling, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroaki","family":"Tateno","sequence":"additional","affiliation":[{"name":"Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Ibaraki 305-8568, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Uno","sequence":"additional","affiliation":[{"name":"Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Hirabayashi","sequence":"additional","affiliation":[{"name":"Research Center for Glycoscience, National Institute of Advanced Industrial Science and Technology, Ibaraki 305-8568, Japan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuzuru","family":"Mikami","sequence":"additional","affiliation":[{"name":"Medical Mycology Research Center, Chiba University, Chiba 260-8673, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Takeda","sequence":"additional","affiliation":[{"name":"Laboratory of Mucosal Immunology, and"},{"name":"Laboratory of Immune Regulation, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuo","family":"Akira","sequence":"additional","affiliation":[{"name":"Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan;"},{"name":"Laboratory of Host Defence,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Saito","sequence":"additional","affiliation":[{"name":"Laboratory for Cell Signaling, RIKEN Research Center for Allergy and Immunology, Yokohama, Kanagawa 230-0045, Japan;"},{"name":"Laboratory of Cell Signaling, World Premier International Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2009,2,10]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"crossref","first-page":"5039","DOI":"10.4049\/jimmunol.163.9.5039","article-title":"A novel LPS-inducible C-type lectin is a transcriptional target of NF-IL6 in macrophages","volume":"163","author":"Matsumoto M","year":"1999","unstructured":"M Matsumoto, et al., A novel LPS-inducible C-type lectin is a transcriptional target of NF-IL6 in macrophages. 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