{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T18:12:32Z","timestamp":1790273552902,"version":"4.1.0"},"reference-count":30,"publisher":"National Academy of Sciences","issue":"34","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,8,26]]},"abstract":"<jats:p>\n                    Engineered mice play an ever-increasing role in defining connections between genotype and phenotypic expression. The potential of magnetic resonance microscopy (MRM) for morphologic phenotyping in the mouse has previously been demonstrated; however, applications have been limited by long scan times, availability of the technology, and a foundation of normative data. This article describes an integrated environment for high-resolution study of normal, transgenic, and mutant mouse models at embryonic and neonatal stages. Three-dimensional images are shown at an isotropic resolution of 19.5 \u03bcm (voxel volumes of 8 pL), acquired in 3 h at embryonic days 10.5\u201319.5 (10 stages) and postnatal days 0\u201332 (6 stages). A web-accessible atlas encompassing this data was developed, and for critical stages of embryonic development (prenatal days 14.5\u201318.5), &gt;200 anatomical structures have been identified and labeled. Also, matching optical histology and analysis tools are provided to compare multiple specimens at multiple developmental stages. The utility of the approach is demonstrated in characterizing cardiac septal defects in conditional mutant embryos lacking the\n                    <jats:italic>Smoothened<\/jats:italic>\n                    receptor gene. Finally, a collaborative paradigm is presented that allows sharing of data across the scientific community. This work makes magnetic resonance microscopy of the mouse embryo and neonate broadly available with carefully annotated normative data and an extensive environment for collaborations.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0805747105","type":"journal-article","created":{"date-parts":[[2008,8,19]],"date-time":"2008-08-19T23:52:10Z","timestamp":1219189930000},"page":"12331-12336","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":109,"title":["High-resolution magnetic resonance histology of the embryonic and neonatal mouse: A 4D atlas and morphologic database"],"prefix":"10.1073","volume":"105","author":[{"given":"Alexandra E.","family":"Petiet","sequence":"first","affiliation":[{"name":"*Center for In Vivo Microscopy and"},{"name":"Department of Biomedical Engineering, Duke University, Box 90281, Durham, NC 27708;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew H.","family":"Kaufman","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh EH8 9XD, United Kingdom; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew M.","family":"Goddeeris","sequence":"additional","affiliation":[{"name":"Departments of \u00a7Cell Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Brandenburg","sequence":"additional","affiliation":[{"name":"*Center for In Vivo Microscopy and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan A.","family":"Elmore","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pathology, National Institute of Envrionmental Health Sciences, Box 12233, Maildrop B3-06, 111 T.W. Alexander Drive, Research Triangle Park, NC 27709"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. Allan","family":"Johnson","sequence":"additional","affiliation":[{"name":"*Center for In Vivo Microscopy and"},{"name":"Radiology, Duke University Medical Center, Box 3302, Durham, NC 27710;"},{"name":"Department of Biomedical Engineering, Duke University, Box 90281, Durham, NC 27708;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2008,8,26]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/242190a0"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1103\/PhysRevB.12.3618"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/322190a0"},{"key":"e_1_3_3_4_2","first-page":"129","article-title":"Nuclear magnetic resonance imaging at microscopic resolution","volume":"68","author":"Johnson GA","year":"1986","unstructured":"GA Johnson, MB Thompson, SL Gewalt, CE Hayes, Nuclear magnetic resonance imaging at microscopic resolution. 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