{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:29:29Z","timestamp":1791268169409,"version":"4.1.0"},"reference-count":48,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,2,3]]},"abstract":"<jats:p>\n                    The CD5 lymphocyte surface receptor is a group B member of the ancient and highly conserved scavenger receptor cysteine-rich superfamily. CD5 is expressed on mature T and B1a cells, where it is known to modulate lymphocyte activation and\/or differentiation processes. Recently, the interaction of a few group B SRCR members (CD6, Sp\u03b1, and DMBT1) with conserved microbial structures has been reported. Protein binding assays presented herein indicate that the CD5 ectodomain binds to and aggregates fungal cells (\n                    <jats:italic>Schizosaccharomyces pombe<\/jats:italic>\n                    ,\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , and\n                    <jats:italic>Cryptococcus neoformans<\/jats:italic>\n                    ) but not to Gram-negative (\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    ) or Gram-positive (\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    ) bacteria. Accordingly, the CD5 ectodomain binds to zymosan but not to purified bacterial cell wall constituents (LPS, lipotheicoic acid, or peptidoglycan), and such binding is specifically competed by \u03b2-glucan but not by mannan. The\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>d<\/jats:sub>\n                    of the rshCD5\/(1\u21923)-\u03b2-\n                    <jats:sc>d<\/jats:sc>\n                    -glucan phosphate interaction is 3.7 \u00b1 0.2 nM as calculated from tryptophan fluorescence data analysis of free and bound rshCD5. Moreover, zymosan binds to membrane-bound CD5, and this induces both MAPK activation and cytokine release. In vivo validation of the fungal binding properties of the CD5 ectodomain is deduced from its protective effect in a mouse model of zymosan-induced septic shock-like syndrome. In conclusion, the present results indicate that the CD5 lymphocyte receptor may sense the presence of conserved fungal components [namely, (1\u21923)-\u03b2-\n                    <jats:sc>d<\/jats:sc>\n                    -glucans] and support the therapeutic potential of soluble CD5 forms in fungal sepsis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0805846106","type":"journal-article","created":{"date-parts":[[2009,1,13]],"date-time":"2009-01-13T21:00:08Z","timestamp":1231880408000},"page":"1506-1511","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":138,"title":["The CD5 ectodomain interacts with conserved fungal cell wall components and protects from zymosan-induced septic shock-like syndrome"],"prefix":"10.1073","volume":"106","author":[{"given":"Jorge","family":"Vera","sequence":"first","affiliation":[{"name":"Servei d'Immunologia, Hospital Cl\u00ednic de Barcelona, Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer, 08036 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael","family":"Fenutr\u00eda","sequence":"additional","affiliation":[{"name":"Servei d'Immunologia, Hospital Cl\u00ednic de Barcelona, Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer, 08036 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga","family":"Ca\u00f1adas","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica y Biolog\u00eda Molecular I, Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Respiratorias, Universidad Complutense de Madrid, 28040 Madrid, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maite","family":"Figueras","sequence":"additional","affiliation":[{"name":"Servei d'Immunologia, Hospital Cl\u00ednic de Barcelona, Institut d'Investigacions Biom\u00e8diques August Pi i Sunyer, 08036 Barcelona, Spain;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rub\u00e9n","family":"Mota","sequence":"additional","affiliation":[{"name":"Servicio de Inmunolog\u00eda, Institut Municipal d'Investigacions M\u00e8diques, Hospital del Mar, 08003 Barcelona, Spain; 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