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Specific visualization of the early-activated cerebral endothelium would provide a powerful tool for the presymptomatic diagnosis of brain disease and evaluation of new therapies. Here, we present the design, construction and in vivo application of carbohydrate-functionalized nanoparticles that allow direct detection of endothelial markers E-\/P-selectin (CD62E\/CD62P) in acute inflammation. These first examples of MRI-visible glyconanoparticles display multiple copies of the natural complex glycan ligand of selectins. Their resulting sensitivity and binding selectivity has allowed acute detection of disease in mammals with beneficial implications for treatment of an expanding patient population suffering from neurological disease.<\/jats:p>","DOI":"10.1073\/pnas.0806787106","type":"journal-article","created":{"date-parts":[[2008,12,23]],"date-time":"2008-12-23T21:48:53Z","timestamp":1230068933000},"page":"18-23","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":223,"title":["Glyconanoparticles allow pre-symptomatic in vivo imaging of brain disease"],"prefix":"10.1073","volume":"106","author":[{"given":"Sander I.","family":"van Kasteren","sequence":"first","affiliation":[{"name":"Department of Chemistry, Chemistry Research Laboratory, University of Oxford, Mansfield Road, Oxford, OX1 3TA, United Kingdom;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra J.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Oxford, Mansfield Road, Oxford, OX1 3QT, United Kingdom; 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