{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:17:46Z","timestamp":1790878666280,"version":"4.1.0"},"reference-count":23,"publisher":"National Academy of Sciences","issue":"38","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2008,9,23]]},"abstract":"<jats:p>\n                    Extensive chemical analyses of spider venoms from many species have revealed complex mixtures of biologically active compounds, of which several have provided important leads for drug development. We have recently shown that NMR spectroscopy can be used advantageously for a direct structural characterization of the small-molecule content of such complex mixtures. Here, we report the application of this strategy to a larger-scale analysis of a collection of spider venoms representing &gt;70 species, which, in combination with mass spectrometric analyses, allowed the identification of a wide range of known, and several previously undescribed, small molecules. These include polyamines, common neurotransmitters, and amino acid derivatives as well as two additional members of a recently discovered family of natural products, the sulfated nucleosides. In the case of the well studied brown recluse spider,\n                    <jats:italic>Loxosceles reclusa<\/jats:italic>\n                    , sulfated guanosine derivatives were found to comprise the major small-molecule components of the venom.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0806840105","type":"journal-article","created":{"date-parts":[[2008,9,15]],"date-time":"2008-09-15T21:39:29Z","timestamp":1221514769000},"page":"14283-14287","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":69,"title":["NMR-spectroscopic screening of spider venom reveals sulfated nucleosides as major components for the brown recluse and related species"],"prefix":"10.1073","volume":"105","author":[{"given":"Frank C.","family":"Schroeder","sequence":"first","affiliation":[{"name":"*Boyce Thompson Institute, Cornell University, Tower Road, Ithaca, NY 14853;"},{"name":"Departments of \u2020Chemistry and Chemical Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew E.","family":"Taggi","sequence":"additional","affiliation":[{"name":"Departments of \u2020Chemistry and Chemical Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Gronquist","sequence":"additional","affiliation":[{"name":"Department of Chemistry, SUNY Fredonia, Fredonia, NY 14063; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rabia U.","family":"Malik","sequence":"additional","affiliation":[{"name":"Departments of \u2020Chemistry and Chemical Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacqualine B.","family":"Grant","sequence":"additional","affiliation":[{"name":"School of Forest Resources and Environmental Science, Michigan Technological University, Houghton, MI 49931"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Eisner","sequence":"additional","affiliation":[{"name":"**Neurobiology and Behavior, Cornell University, Ithaca, NY 14853;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerrold","family":"Meinwald","sequence":"additional","affiliation":[{"name":"Departments of \u2020Chemistry and Chemical Biology and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2008,9,23]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.2174\/157341105774573901"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmr.2005.08.016"},{"key":"e_1_3_3_3_2","first-page":"511","article-title":"Single-insect NMR: A new tool to probe chemical biodiversity","volume":"1","author":"Dossey AT","year":"2006","unstructured":"AT Dossey, SS Walse, JR Rocca, AS Edison, Single-insect NMR: A new tool to probe chemical biodiversity. 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