{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T23:54:09Z","timestamp":1792799649946,"version":"4.5.0"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"4","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,1,27]]},"abstract":"<jats:p>\n                    TRPA1 functions as an excitatory ionotropic receptor in sensory neurons. It was originally described as a noxious cold-activated channel, but its cold sensitivity has been disputed in later studies, and the contribution of TRPA1 to thermosensing is currently a matter of strong debate. Here, we provide several lines of evidence to establish that TRPA1 acts as a cold sensor in vitro and in vivo. First, we demonstrate that heterologously expressed TRPA1 is activated by cold in a Ca\n                    <jats:sup>2+<\/jats:sup>\n                    -independent and Ca\n                    <jats:sup>2+<\/jats:sup>\n                    store-independent manner; temperature-dependent gating of TRPA1 is mechanistically analogous to that of other temperature-sensitive TRP channels, and it is preserved after treatment with the TRPA1 agonist mustard oil. Second, we identify and characterize a specific subset of cold-sensitive trigeminal ganglion neurons that is absent in TRPA1-deficient mice. Finally, cold plate and tail-flick experiments reveal TRPA1-dependent, cold-induced nociceptive behavior in mice. We conclude that TRPA1 acts as a major sensor for noxious cold.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0808487106","type":"journal-article","created":{"date-parts":[[2009,1,14]],"date-time":"2009-01-14T22:42:49Z","timestamp":1231972969000},"page":"1273-1278","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":535,"title":["TRPA1 acts as a cold sensor in vitro and in vivo"],"prefix":"10.1073","volume":"106","author":[{"given":"Yuji","family":"Karashima","sequence":"first","affiliation":[{"name":"Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell Biology, Campus Gasthuisberg O&amp;N1, Katholieke Universiteit Leuven, Herestraat 49 bus 802, B-3000 Leuven, Belgium;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karel","family":"Talavera","sequence":"additional","affiliation":[{"name":"Laboratory of Ion Channel Research, Division of Physiology, Department of Molecular Cell 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