{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T08:53:49Z","timestamp":1790326429725,"version":"4.1.0"},"reference-count":27,"publisher":"National Academy of Sciences","issue":"8","license":[{"start":{"date-parts":[[2009,8,24]],"date-time":"2009-08-24T00:00:00Z","timestamp":1251072000000},"content-version":"vor","delay-in-days":181,"URL":"http:\/\/www.pnas.org\/site\/misc\/userlicense.xhtml"}],"content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,2,24]]},"abstract":"<jats:p>\n                    Anti-p200 pemphigoid has been characterized by autoantibodies to an unidentified 200-kDa protein (p200) of the dermal\u2212epidermal junction. The objective of this study was to identify p200. We performed 2D gel electrophoresis of dermal extracts and immunoblotting with patients' sera, followed by MS analysis of a unique protein band. The protein band corresponded to laminin \u03b31. Anti-laminin \u03b31 mAb reacted with the anti-p200 immunoprecipitates by immunoblotting. Sera from 32 patients with anti-p200 pemphigoid showed 90% reactivity to the recombinant products of laminin \u03b31. None of the healthy control sera reacted with laminin \u03b31. By immunoblotting, reactivity of a patient's serum with p200 was competitively inhibited by adding anti-laminin \u03b31\n                    <jats:italic>C<\/jats:italic>\n                    -terminus mAb. Purified anti-p200 IgG also inhibited the reactivity of this mAb to dermal laminin \u03b31. Most laminin \u03b31-positive sera showed reactivity with recombinant laminin \u03b31\n                    <jats:italic>C<\/jats:italic>\n                    -terminal E8 fragment. Reactivity of patients' sera and purified IgG to dermal laminin \u03b31 was higher than reactivity to blood vessel laminin \u03b31 under reducing conditions. These results suggest that laminin \u03b31 is the autoantigen for patients with anti-p200 pemphigoid. The autoantibodies may specifically recognize dermal laminin \u03b31 with unique posttranslational modifications. The epitope is localized to the 246\n                    <jats:italic>C<\/jats:italic>\n                    -terminal amino acids within the coiled-coil domain. The 9\n                    <jats:italic>C<\/jats:italic>\n                    -terminal residues are known to be critically involved in laminin recognition by integrins.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0809230106","type":"journal-article","created":{"date-parts":[[2009,2,7]],"date-time":"2009-02-07T00:06:11Z","timestamp":1233965171000},"page":"2800-2805","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":156,"title":["Anti-laminin gamma-1 pemphigoid"],"prefix":"10.1073","volume":"106","author":[{"given":"Teruki","family":"Dainichi","sequence":"first","affiliation":[{"name":"Department of Dermatology, Kurume University School of Medicine, Fukuoka 830-0011, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadamu","family":"Kurono","sequence":"additional","affiliation":[{"name":"Iberica Holdings Company, Limited, Kurume University Translational Research Center, Fukuoka 812-0011, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bungo","family":"Ohyama","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Kurume University School of Medicine, Fukuoka 830-0011, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norito","family":"Ishii","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Kurume University School of Medicine, Fukuoka 830-0011, Japan;"},{"name":"Department of Dermatology, University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriko","family":"Sanzen","sequence":"additional","affiliation":[{"name":"Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chisei","family":"Shimono","sequence":"additional","affiliation":[{"name":"Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukimasa","family":"Taniguchi","sequence":"additional","affiliation":[{"name":"Laboratory of Extracellular Matrix Biochemistry, Institute for Protein Research, Osaka University, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Koga","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Kurume University School of Medicine, Fukuoka 830-0011, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tadashi","family":"Karashima","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Kurume University School of Medicine, Fukuoka 830-0011, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichiro","family":"Yasumoto","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Kurume University School of Medicine, Fukuoka 830-0011, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Detlef","family":"Zillikens","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of L\u00fcbeck, 23538 L\u00fcbeck, Germany; 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