{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T15:21:23Z","timestamp":1791559283765,"version":"4.3.4"},"reference-count":42,"publisher":"National Academy of Sciences","issue":"14","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,4,7]]},"abstract":"<jats:p>Although somatic cell nuclear transfer (SCNT) cloning is more efficient in cattle than in any other species tested so far, there is a high rate of pregnancy failure that has been linked to structural and functional abnormalities of the placenta. We tested the hypothesis that these changes may originate from disturbed embryo\u2013maternal interactions in the peri-implantation period. Therefore, we evaluated the response of the endometrium to SCNT embryos (produced from 7 different fetal fibroblast cell lines) as compared with embryos derived from in vitro fertilization (IVF). SCNT embryos and IVF embryos were cultured under identical conditions to the blastocyst stage (day 7) and were transferred to corresponding recipients, which were slaughtered at day 18 of pregnancy. The mRNA profiles of endometrium samples were obtained using a custom cDNA microarray enriched for transcripts differentially expressed in the endometrium and\/or oviduct epithelium during the estrous cycle and\/or early pregnancy. Overall, the variation in mRNA profiles was greater in the SCNT group than in the IVF group. Furthermore, 58 transcripts were differentially abundant in endometria from SCNT and IVF pregnancies. Prominent examples are orphan nuclear receptor COUP-TFII and connexin 43, both known to play important roles in uterine receptivity and conceptus placentation. These findings suggest that placental failure in bovine clone pregnancies may originate from abnormal embryo\u2013maternal communication that develops during the peri-implantation period. Endometrium transcriptome profiles may serve as a tool to evaluate SCNT embryos for their ability to establish pregnancy and develop a functional placenta.<\/jats:p>","DOI":"10.1073\/pnas.0811841106","type":"journal-article","created":{"date-parts":[[2009,3,23]],"date-time":"2009-03-23T21:30:41Z","timestamp":1237843841000},"page":"5681-5686","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":190,"title":["The endometrium responds differently to cloned versus fertilized embryos"],"prefix":"10.1073","volume":"106","author":[{"given":"Stefan","family":"Bauersachs","sequence":"first","affiliation":[{"name":"Molecular Animal Breeding and Biotechnology, and"},{"name":"Laboratory for Functional Genome Analysis, Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne E.","family":"Ulbrich","sequence":"additional","affiliation":[{"name":"Physiology Weihenstephan, Technische Universit\u00e4t M\u00fcnchen, 85354 Freising, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valeri","family":"Zakhartchenko","sequence":"additional","affiliation":[{"name":"Molecular Animal Breeding and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Minten","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&amp;M University, College Station, TX 77843-2471; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myriam","family":"Reichenbach","sequence":"additional","affiliation":[{"name":"Molecular Animal Breeding and Biotechnology, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Horst-Dieter","family":"Reichenbach","sequence":"additional","affiliation":[{"name":"Bavarian State Institute for Agriculture, Institute for Animal Breeding, 85586 Poing, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helmut","family":"Blum","sequence":"additional","affiliation":[{"name":"Laboratory for Functional Genome Analysis, Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Spencer","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Texas A&amp;M University, College Station, TX 77843-2471; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eckhard","family":"Wolf","sequence":"additional","affiliation":[{"name":"Molecular Animal Breeding and Biotechnology, and"},{"name":"Laboratory for Functional Genome Analysis, Gene Center, Ludwig-Maximilians-Universit\u00e4t M\u00fcnchen, 81377 Munich, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2009,4,7]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/385810a0"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.278.5346.2130"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/35016604"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.09.036"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.241522698"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(03)00419-6"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-0436.2003.710201.x"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod66.1.6"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.103.020982"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.20506\/rst.24.1.1566"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod60.4.996"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.6.1787"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2005.7.238"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1089\/clo.2005.7.289"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.theriogenology.2006.01.061"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1530\/rep.1.01217"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.043919"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2007-0132"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308487101"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1677\/jme.1.01799"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.046748"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0897.2007.00512.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1530\/REP-07-0327"},{"key":"e_1_3_3_24_2","first-page":"233","article-title":"Mx proteins: GTPases involved in the interferon-induced antiviral state","volume":"176","author":"Pavlovic J","year":"1993","unstructured":"J Pavlovic, A Schroder, A Blank, F Pitossi, P Staeheli, Mx proteins: GTPases involved in the interferon-induced antiviral state. 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