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The most stable complexes induce tetramer formation of IN, as happens during the physiological integration reaction, and are able to catalyze the strand transfer step of retroviral integration. One of these complexes also binds strand-transfer inhibitors of HIV antiviral drugs, making it uniquely valuable among the mutants of this set for understanding portions of the integration reaction. This novel complex may help define substrate interactions and delineate the mechanism of action of known integration inhibitors.<\/jats:p>","DOI":"10.1073\/pnas.0811919106","type":"journal-article","created":{"date-parts":[[2009,5,4]],"date-time":"2009-05-04T21:54:30Z","timestamp":1241474070000},"page":"8192-8197","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":30,"title":["Catalytically-active complex of HIV-1 integrase with a viral DNA substrate binds anti-integrase drugs"],"prefix":"10.1073","volume":"106","author":[{"given":"Akram","family":"Alian","sequence":"first","affiliation":[{"name":"Departments of aBiochemistry and Biophysics,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah L.","family":"Griner","sequence":"additional","affiliation":[{"name":"Departments of aBiochemistry and Biophysics,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicki","family":"Chiang","sequence":"additional","affiliation":[{"name":"Laboratory Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Tsiang","sequence":"additional","affiliation":[{"name":"Gilead Sciences, Foster City, CA 94404"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregg","family":"Jones","sequence":"additional","affiliation":[{"name":"Gilead Sciences, Foster City, CA 94404"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabriel","family":"Birkus","sequence":"additional","affiliation":[{"name":"Gilead Sciences, Foster City, CA 94404"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Romas","family":"Geleziunas","sequence":"additional","affiliation":[{"name":"Gilead Sciences, Foster City, CA 94404"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew D.","family":"Leavitt","sequence":"additional","affiliation":[{"name":"Laboratory Medicine, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"Stroud","sequence":"additional","affiliation":[{"name":"Departments of aBiochemistry and Biophysics,"},{"name":"Pharmaceutical Chemistry, University of California, San Francisco, CA 94158; 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