{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T20:19:36Z","timestamp":1790194776981,"version":"4.1.0"},"reference-count":40,"publisher":"National Academy of Sciences","issue":"5","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,2,3]]},"abstract":"<jats:p>\n                    The gene networks underlying closure of the optic fissure during vertebrate eye development are poorly understood. Here, we profile global gene expression during optic fissure closure using laser capture microdissected (LCM) tissue from the margins of the fissure. From these data, we identify a unique role for the C\n                    <jats:sub>2<\/jats:sub>\n                    H\n                    <jats:sub>2<\/jats:sub>\n                    zinc finger proteins Nlz1 and Nlz2 in normal fissure closure. Gene knockdown of\n                    <jats:italic>nlz1<\/jats:italic>\n                    and\/or\n                    <jats:italic>nlz2<\/jats:italic>\n                    in zebrafish leads to a failure of the optic fissure to close, a phenotype which closely resembles that seen in human uveal coloboma. We also identify misregulation of\n                    <jats:italic>pax2<\/jats:italic>\n                    in the developing eye of morphant fish, suggesting that Nlz1 and Nlz2 act upstream of the\n                    <jats:italic>Pax2<\/jats:italic>\n                    pathway in directing proper closure of the optic fissure.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0812017106","type":"journal-article","created":{"date-parts":[[2009,1,26]],"date-time":"2009-01-26T20:45:51Z","timestamp":1233002751000},"page":"1462-1467","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":72,"title":["Expression profiling during ocular development identifies 2\n                    <i>Nlz<\/i>\n                    genes with a critical role in optic fissure closure"],"prefix":"10.1073","volume":"106","author":[{"given":"Jacob D.","family":"Brown","sequence":"first","affiliation":[{"name":"Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;"},{"name":"Biochemistry and Molecular &amp; Cellular Biology, Georgetown University, 3900 Reservoir Road NW, Washington, DC 20007;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sunit","family":"Dutta","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kapil","family":"Bharti","sequence":"additional","affiliation":[{"name":"Mammalian Development Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Bonner","sequence":"additional","affiliation":[{"name":"Section on Medical Biophysics, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Munson","sequence":"additional","affiliation":[{"name":"Mathematical and Statistical Computing Laboratory, Center for Information Technology, National Institutes of Health, Bethesda, MD 20892; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor B.","family":"Dawid","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Genetics,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amana L.","family":"Akhtar","sequence":"additional","affiliation":[{"name":"Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ighovie F.","family":"Onojafe","sequence":"additional","affiliation":[{"name":"Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ramakrishna P.","family":"Alur","sequence":"additional","affiliation":[{"name":"Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey M.","family":"Gross","sequence":"additional","affiliation":[{"name":"Molecular Cell &amp; Developmental Biology, University of Texas at Austin, 1 University Station C1000, Austin, TX 78712-0195"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Fielding","family":"Hejtmancik","sequence":"additional","affiliation":[{"name":"Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Jiao","sequence":"additional","affiliation":[{"name":"Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wai-Yee","family":"Chan","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular &amp; Cellular Biology, Georgetown University, 3900 Reservoir Road NW, Washington, DC 20007;"},{"name":"Section on Developmental Genetics, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian P.","family":"Brooks","sequence":"additional","affiliation":[{"name":"Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD 20892;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2009,2,3]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"1","article-title":"The early development of the eye in staged human embryos","author":"O'Rahilly","year":"1966","unstructured":"O'Rahilly, The early development of the eye in staged human embryos. 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