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We demonstrate that the spliceosome pairs exons with an astonishingly high degree of accuracy that may be limited by the quality of pre-mRNAs generated by RNA pol II. The error rate of exon pairing is increased by the effects of the neurodegenerative disorder spinal muscular atrophy because of reduced levels of\n                    <jats:italic>Survival of Motor Neuron<\/jats:italic>\n                    , a master assembler of spliceosomal components. We conclude that all multi-intron-containing genes are alternatively spliced and that the reduction of SMN results in a general splicing defect that is mediated through alterations in the fidelity of splice-site pairing.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0813128106","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T20:34:16Z","timestamp":1233261256000},"page":"1766-1771","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":82,"title":["Splice-site pairing is an intrinsically high fidelity process"],"prefix":"10.1073","volume":"106","author":[{"given":"Kristi L.","family":"Fox-Walsh","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697-4025"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klemens J.","family":"Hertel","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, University of California, Irvine, CA 92697-4025"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2009,2,10]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"525","volume-title":"The RNA World","author":"Burge CB","year":"1999","unstructured":"CB Burge, T Tuschl, PA Sharp, Splicing of precursors to mRNAs by the spliceosome. 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