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A major experimental advance was the cell culture-based scrapie cell assay, allowing prion titres to be estimated more precisely and an order of magnitude faster than by animal bioassays. Here we describe a bioassay method that exploits the marked binding affinity of prions to steel surfaces. Using steel wires as a concentrating and sensitization tool and combining with an adapted scrapie cell endpoint assay we can achieve, for mouse prions, a sensitivity 100\u00d7 higher than that achieved in standard mouse bioassays. The rapidity and sensitivity of this assay offers a major advance over small animal bioassay in many aspects of prion research. In addition, its specific application in assay of metal-bound prions allows evaluation of novel prion decontamination methods.<\/jats:p>","DOI":"10.1073\/pnas.0813342106","type":"journal-article","created":{"date-parts":[[2009,2,9]],"date-time":"2009-02-09T21:10:18Z","timestamp":1234213818000},"page":"3479-3483","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":58,"title":["Highly sensitive, quantitative cell-based assay for prions adsorbed to solid surfaces"],"prefix":"10.1073","volume":"106","author":[{"given":"Julie Ann","family":"Edgeworth","sequence":"first","affiliation":[{"name":"Medical Research Council Prion Unit, Department of Neurodegenerative Disease, University College London Institute of Neurology, Queen Square, London WC1N 3BG, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graham S.","family":"Jackson","sequence":"additional","affiliation":[{"name":"Medical Research Council Prion 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