{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T14:51:00Z","timestamp":1781103060032,"version":"3.54.1"},"reference-count":43,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,4,16]]},"abstract":"<jats:p>\n                    Cerebral vasospasm and ischemic damage are important causes of mortality and morbidity in patients affected by aneurysmal subarachnoid hemorrhage (SAH). Recently, i.p. administration of recombinant human erythropoietin (r-Hu-EPO) has been shown to exert a neuroprotective effect during experimental SAH. The present study was conducted to evaluate further the effect of r-Hu-EPO administration after SAH in rabbits on neurological outcome, degree of basilar artery spasm, and magnitude of neuronal ischemic damage. Experimental animals were divided into six groups: group 1 (\n                    <jats:italic>n<\/jats:italic>\n                    = 8), control; group 2 (\n                    <jats:italic>n<\/jats:italic>\n                    = 8), control plus placebo; group 3 (\n                    <jats:italic>n<\/jats:italic>\n                    = 8), control plus r-Hu-EPO; group 4 (\n                    <jats:italic>n<\/jats:italic>\n                    = 8), SAH; group 5 (\n                    <jats:italic>n<\/jats:italic>\n                    = 8), SAH plus placebo; group 6 (\n                    <jats:italic>n<\/jats:italic>\n                    = 8), SAH plus r-Hu-EPO. r-Hu-EPO, at a dose of 1,000 units\/kg, and placebo were injected i.p. starting 5 min after inducing SAH and followed by clinical and pathological assessment 72 h later. Systemic administration of r-Hu-EPO produced significant increases in cerebrospinal fluid EPO concentrations (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), and reduced vasoconstriction of the basilar artery (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05), ischemic neuronal damage (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.001), and subsequent neurological deterioration (\n                    <jats:italic>P<\/jats:italic>\n                    &lt; 0.05). These observations suggest that r-Hu-EPO may provide an effective treatment to reduce the post-SAH morbidity.\n                  <\/jats:p>","DOI":"10.1073\/pnas.082097299","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:45:09Z","timestamp":1027680309000},"page":"5627-5631","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":152,"title":["Beneficial effects of systemic administration of recombinant human erythropoietin in rabbits subjected to subarachnoid hemorrhage"],"prefix":"10.1073","volume":"99","author":[{"given":"Giovanni","family":"Grasso","sequence":"first","affiliation":[{"name":"Departments of Neurosurgery, Internal Medicine, Veterinary Pathology, and Pharmacology, University of Messina, 98122 Messina, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Buemi","sequence":"additional","affiliation":[{"name":"Departments of Neurosurgery, Internal 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