{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T22:35:18Z","timestamp":1791585318810,"version":"4.3.4"},"reference-count":45,"publisher":"National Academy of Sciences","issue":"9","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,4,30]]},"abstract":"<jats:p>\n                    A key mechanism underlying physiological angiogenesis of the human endometrium is its ability to regenerate the vascular capillary network and to perform vascular remodeling (i.e., development of spiral arteries). Vascular endothelial growth factor (VEGF) is associated with angiogenesis and capillary permeability in this tissue. VEGF is expressed as several spliced variants, its main human isoforms contain 121 and 165 aa; 17\u03b2-estradiol (E\n                    <jats:sub>2<\/jats:sub>\n                    ) increases endometrial VEGF, possibly in all isoforms. Here we show that progesterone (P) selectively increases the expression of the VEGF\n                    <jats:sub>189<\/jats:sub>\n                    (V\n                    <jats:sub>189<\/jats:sub>\n                    ) isoform in the human uterus. V\n                    <jats:sub>189<\/jats:sub>\n                    is identified in the conditioned medium of stromal cells treated with E\n                    <jats:sub>2<\/jats:sub>\n                    + P; its presence in this\n                    <jats:italic>in vitro<\/jats:italic>\n                    model of decidual stromal cells is detected after 6\u20138 days, using ELISA, and after 8\u201310 days, using Western blot analysis with different antibodies, including one specific for V\n                    <jats:sub>189<\/jats:sub>\n                    . The secretion pattern of V\n                    <jats:sub>189<\/jats:sub>\n                    parallels that of the decidual protein IGFBP-1. V\n                    <jats:sub>189<\/jats:sub>\n                    is secreted as a native isoform, as compared with the migration of recombinant V\n                    <jats:sub>189<\/jats:sub>\n                    by SDS\/PAGE.\n                    <jats:italic>In situ<\/jats:italic>\n                    hybridization and immunocytochemistry\n                    <jats:sub>,<\/jats:sub>\n                    performed on the same biopsies, suggest that decidual cells express V\n                    <jats:sub>189<\/jats:sub>\n                    during the mid-late secretory phase of the menstrual cycle and early gestation. Finally, using an\n                    <jats:italic>in vivo<\/jats:italic>\n                    permeability assay, we show that native V\n                    <jats:sub>189<\/jats:sub>\n                    increases capillary permeability. These observations demonstrate that P regulates V\n                    <jats:sub>189<\/jats:sub>\n                    expression in decidual cells, which could have important implications for understanding uterine vascular remodeling and implantation, and may be relevant in a range of disease states such as edema and irregular bleeding.\n                  <\/jats:p>","DOI":"10.1073\/pnas.082110999","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:46:49Z","timestamp":1027680409000},"page":"6023-6028","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":87,"title":["A dynamic shift of VEGF isoforms with a transient and selective progesterone-induced expression of VEGF\n                    <sub>189<\/sub>\n                    regulates angiogenesis and vascular permeability in human uterus"],"prefix":"10.1073","volume":"99","author":[{"given":"Magali","family":"Ancelin","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U553, \u201cH\u00e9mostase, Endoth\u00e9lium et Angiog\u00e9n\u00e8se,\u201d H\u00f4pital Saint Louis\/B\u00e2t INSERM, 1 Avenue C. Vellefaux, 75010 Paris, France; INSERM U460, \u201cRemodelage Cardiovasculaire,\u201d CHU X. Bichat, 16 Rue H. Huchard, 75018 Paris, France; and Unit\u00e9 Mixte de Recherche, Centre National de la Recherche Scientifique (CNRS) 5089, Institut de Pharmacologie et Biologie structurale, 205 Route de Narbonne, 31077 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Buteau-Lozano","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U553, \u201cH\u00e9mostase, Endoth\u00e9lium et Angiog\u00e9n\u00e8se,\u201d H\u00f4pital Saint Louis\/B\u00e2t INSERM, 1 Avenue C. Vellefaux, 75010 Paris, France; INSERM U460, \u201cRemodelage Cardiovasculaire,\u201d CHU X. Bichat, 16 Rue H. Huchard, 75018 Paris, France; and Unit\u00e9 Mixte de Recherche, Centre National de la Recherche Scientifique (CNRS) 5089, Institut de Pharmacologie et Biologie structurale, 205 Route de Narbonne, 31077 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geri","family":"Meduri","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U553, \u201cH\u00e9mostase, Endoth\u00e9lium et Angiog\u00e9n\u00e8se,\u201d H\u00f4pital Saint Louis\/B\u00e2t INSERM, 1 Avenue C. Vellefaux, 75010 Paris, France; INSERM U460, \u201cRemodelage Cardiovasculaire,\u201d CHU X. Bichat, 16 Rue H. Huchard, 75018 Paris, France; and Unit\u00e9 Mixte de Recherche, Centre National de la Recherche Scientifique (CNRS) 5089, Institut de Pharmacologie et Biologie structurale, 205 Route de Narbonne, 31077 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Osborne-Pellegrin","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U553, \u201cH\u00e9mostase, Endoth\u00e9lium et Angiog\u00e9n\u00e8se,\u201d H\u00f4pital Saint Louis\/B\u00e2t INSERM, 1 Avenue C. Vellefaux, 75010 Paris, France; INSERM U460, \u201cRemodelage Cardiovasculaire,\u201d CHU X. Bichat, 16 Rue H. Huchard, 75018 Paris, France; and Unit\u00e9 Mixte de Recherche, Centre National de la Recherche Scientifique (CNRS) 5089, Institut de Pharmacologie et Biologie structurale, 205 Route de Narbonne, 31077 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Sordello","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U553, \u201cH\u00e9mostase, Endoth\u00e9lium et Angiog\u00e9n\u00e8se,\u201d H\u00f4pital Saint Louis\/B\u00e2t INSERM, 1 Avenue C. Vellefaux, 75010 Paris, France; INSERM U460, \u201cRemodelage Cardiovasculaire,\u201d CHU X. Bichat, 16 Rue H. Huchard, 75018 Paris, France; and Unit\u00e9 Mixte de Recherche, Centre National de la Recherche Scientifique (CNRS) 5089, Institut de Pharmacologie et Biologie structurale, 205 Route de Narbonne, 31077 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"Plou\u00ebt","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U553, \u201cH\u00e9mostase, Endoth\u00e9lium et Angiog\u00e9n\u00e8se,\u201d H\u00f4pital Saint Louis\/B\u00e2t INSERM, 1 Avenue C. Vellefaux, 75010 Paris, France; INSERM U460, \u201cRemodelage Cardiovasculaire,\u201d CHU X. Bichat, 16 Rue H. Huchard, 75018 Paris, France; and Unit\u00e9 Mixte de Recherche, Centre National de la Recherche Scientifique (CNRS) 5089, Institut de Pharmacologie et Biologie structurale, 205 Route de Narbonne, 31077 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Perrot-Applanat","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM) U553, \u201cH\u00e9mostase, Endoth\u00e9lium et Angiog\u00e9n\u00e8se,\u201d H\u00f4pital Saint Louis\/B\u00e2t INSERM, 1 Avenue C. Vellefaux, 75010 Paris, France; INSERM U460, \u201cRemodelage Cardiovasculaire,\u201d CHU X. Bichat, 16 Rue H. Huchard, 75018 Paris, France; and Unit\u00e9 Mixte de Recherche, Centre National de la Recherche Scientifique (CNRS) 5089, Institut de Pharmacologie et Biologie structurale, 205 Route de Narbonne, 31077 Toulouse, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2002,4,23]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"272","volume-title":"Reproductive Endocrinology, Surgery and Technology","author":"Giudice L","year":"1996","unstructured":"L Giudice Reproductive Endocrinology, Surgery and Technology, eds E Y Adashi, J A Rock, Z Rosenwaks (Lippincott\u2013Raven, Philadelphia), pp. 272\u2013300 (1996)."},{"key":"e_1_3_3_2_2","first-page":"157","volume-title":"Encyclopedic Reference of Vascular Biology and Pathology","author":"Perrot-Applanat M","year":"2000","unstructured":"M Perrot-Applanat Encyclopedic Reference of Vascular Biology and Pathology, ed A Bikfalvi (Springer-Verlag, Berlin), pp. 157\u2013162 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