{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:38:35Z","timestamp":1791596315506,"version":"4.3.4"},"reference-count":35,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2002,4,16]]},"abstract":"<jats:p>\n                    Cerebral ischemia stimulates increased activity of polyamine oxidase, a ubiquitous enzyme that catabolizes polyamines to produce 3-aminopropanal. 3-Aminopropanal is a reactive aldehyde that mediates progressive neuronal necrosis and glial apoptosis. Here we report that increased levels of 3-aminopropanal-modified protein levels in humans after aneurysmal subarachnoid hemorrhage correlate with the degree of cerebral injury as measured by admission Hunt\/Hess grade.\n                    <jats:italic>In vitro<\/jats:italic>\n                    screening of clinically approved drugs reveals that\n                    <jats:italic>N<\/jats:italic>\n                    -2-mercaptopropionyl glycine (\n                    <jats:italic>N<\/jats:italic>\n                    -2-MPG), an agent clinically approved for prevention of renal stones in patients with cysteinuria, significantly inhibits the cytotoxicity of 3-aminopropanal.\n                    <jats:italic>N<\/jats:italic>\n                    -2-MPG reacts with 3-aminopropanal to yield a nontoxic thioacetal adduct, as confirmed by electrospray ionization mass spectroscopy. Administration of\n                    <jats:italic>N<\/jats:italic>\n                    -2-MPG in clinically relevant doses to rats significantly reduces cerebral 3-aminopropanal-modified protein immunoreactivity and infarct volume in a standardized model of middle cerebral artery occlusion, even when the agent is administered after the onset of ischemia. These results implicate 3-aminopropanal as a therapeutic target for cerebral ischemia.\n                  <\/jats:p>","DOI":"10.1073\/pnas.082609299","type":"journal-article","created":{"date-parts":[[2002,7,26]],"date-time":"2002-07-26T10:45:09Z","timestamp":1027680309000},"page":"5579-5584","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":76,"title":["Neuroprotection in cerebral ischemia by neutralization of 3-aminopropanal"],"prefix":"10.1073","volume":"99","author":[{"given":"Svetlana","family":"Ivanova","sequence":"first","affiliation":[{"name":"Laboratories of Biomedical Science and Medicinal Chemistry, and Division of Biology and Human Genetics, North Shore-Long Island Jewish Research Institute, Manhasset, NY 11030; Department of Neurological Surgery, College of Physicians and Surgeons, Columbia University, New York, NY 10032; James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202; and Department of Pharmacology, Hebrew University, Jerusalem 91120, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franak","family":"Batliwalla","sequence":"additional","affiliation":[{"name":"Laboratories of Biomedical Science and Medicinal Chemistry, and Division of Biology and Human Genetics, North Shore-Long Island Jewish Research Institute, Manhasset, NY 11030; 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