{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T15:59:22Z","timestamp":1781107162276,"version":"3.54.1"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"16","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,4,21]]},"abstract":"<jats:p>\n                    Antigen expressed as MHC Class I glycoprotein (pMHCI) complexes on dendritic cells is the primary driver of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cell clonal expansion and differentiation. As we seek to define the molecular differences between acutely stimulated cytotoxic T lymphocyte (CTL) effectors and long-lived memory T cells, it is essential that we understand the duration of\n                    <jats:italic>in vivo<\/jats:italic>\n                    pMHCI persistence. Although infectious influenza A virus is readily cleared by mammalian hosts, that does not necessarily mean that all influenza antigen is totally eliminated. An exhaustive series of carefully controlled adoptive transfer experiments using 3 different carboxy fluorescein diacetate succinimidyl ester\u2013labeled T cell receptor\u2013transgenic CTL populations and a spectrum of genetically engineered and wild-type influenza A viruses provided no evidence for pMHCI persistence over the 30\u201360-d interval after virus challenge. Molecular profiles identified in antigen-specific T cells at this time may thus be considered to reflect established immunologic memory and not recent CTL activation from a persistent pMHCI pool.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0901128106","type":"journal-article","created":{"date-parts":[[2009,4,3]],"date-time":"2009-04-03T22:43:05Z","timestamp":1238798585000},"page":"6724-6729","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":13,"title":["Transience of MHC Class I-restricted antigen presentation after influenza A virus infection"],"prefix":"10.1073","volume":"106","author":[{"given":"Justine D.","family":"Mintern","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sammy","family":"Bedoui","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gayle M.","family":"Davey","sequence":"additional","affiliation":[{"name":"Walter and Eliza Hall Institute of Medical Research, Parkville, Victoria 3050, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica M.","family":"Moffat","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter C.","family":"Doherty","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Melbourne, Parkville 3010, Australia;"},{"name":"Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 38105; 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