{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:27:02Z","timestamp":1791264422473,"version":"4.1.0"},"reference-count":41,"publisher":"National Academy of Sciences","issue":"34","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,8,25]]},"abstract":"<jats:p>\n                    Infecting about one-half of the global human population,\n                    <jats:italic>Helicobacter pylori<\/jats:italic>\n                    is well established as the primary cause of gastritis, duodenal ulcer, and gastric cancer. Currently there is no clear information regarding if and how host cells interact with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    , and if such interactions are dependent on the type of gastric disease. Using fluorescently labeled fucose-containing glycoconjugates, we provide evidence observing both the uptake of\n                    <jats:sc>l<\/jats:sc>\n                    -fucose from gastric cancer cells to\n                    <jats:italic>H. pylori<\/jats:italic>\n                    and that human \u03b1-\n                    <jats:sc>l<\/jats:sc>\n                    -fucosidase 2 (FUCA2) is secreted only under coculture conditions (i.e., host cells infected with\n                    <jats:italic>H. pylori<\/jats:italic>\n                    ). Upon depletion of FUCA2 by RNA interference and detection of translocated CagA (a virulence factor of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    ) in host cells, FUCA2 was found to be essential for\n                    <jats:italic>H. pylori<\/jats:italic>\n                    adhesion, in particular to the gastric cancer- and duodenal ulcer-specific strains. Additionally FUCA2 was shown to significantly enhance the expression of Lewis x antigen in\n                    <jats:italic>H. pylori<\/jats:italic>\n                    , which is critical for bacterial cell adhesion in the pathogenesis and defense strategy to escape host surveillance. These findings not only demonstrate an important connection between FUCA2 and the adhesion, growth, and pathogenicity of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    , but also support the idea that FUCA2 is a potential target for clinical diagnosis and therapeutic intervention of\n                    <jats:italic>H. pylori<\/jats:italic>\n                    -related diseases.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0903286106","type":"journal-article","created":{"date-parts":[[2009,8,7]],"date-time":"2009-08-07T21:55:24Z","timestamp":1249682124000},"page":"14581-14586","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":68,"title":["Role for \u03b1-\n                    <scp>l<\/scp>\n                    -fucosidase in the control of\n                    <i>Helicobacter pylori<\/i>\n                    -infected gastric cancer cells"],"prefix":"10.1073","volume":"106","author":[{"given":"Ta-Wei","family":"Liu","sequence":"first","affiliation":[{"name":"Institute of Biological Chemistry and"},{"name":"Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, 10617 Taiwan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Wen","family":"Ho","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry and"},{"name":"Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, No.128 Academia Road Section 2, Nan-Kang, Taipei, 11529, Taiwan;"},{"name":"Department of Chemistry, National Tsing-Hua University, Hsin-Chu, 30013 Taiwan; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Hung","family":"Huang","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry and"},{"name":"Institute of Chemistry, and"},{"name":"Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, 10617 Taiwan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sue-Ming","family":"Chang","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry and"},{"name":"Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, 10617 Taiwan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shide D.","family":"Popat","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Ting","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Chemistry, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming-Shiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology, Department of Internal Medicine, National Taiwan University Hospital, Taipei, 10002 Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Ju","family":"Chen","sequence":"additional","affiliation":[{"name":"The Genomics Research Center,"},{"name":"Institute of Chemistry, and"},{"name":"Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, No.128 Academia Road Section 2, Nan-Kang, Taipei, 11529, Taiwan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chun-Hung","family":"Lin","sequence":"additional","affiliation":[{"name":"Institute of Biological Chemistry and"},{"name":"The Genomics Research Center,"},{"name":"Chemical Biology and Molecular Biophysics, Taiwan International Graduate Program, Academia Sinica, No.128 Academia Road Section 2, Nan-Kang, Taipei, 11529, Taiwan;"},{"name":"Institute of Biochemical Sciences, College of Life Science, National Taiwan University, Taipei, 10617 Taiwan;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2009,8,25]]},"reference":[{"key":"e_1_3_2_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc703"},{"key":"e_1_3_2_2_2","first-page":"196","article-title":"Chronic gastritis and Helicobacter pylori","volume":"12","author":"Cave DR","year":"2001","unstructured":"DR Cave, Chronic gastritis and Helicobacter pylori. 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