{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T22:27:45Z","timestamp":1791325665675,"version":"4.3.1"},"reference-count":52,"publisher":"National Academy of Sciences","issue":"28","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,7,14]]},"abstract":"<jats:p>\n                    Mice lacking the \u03b21-subunit (gene,\n                    <jats:italic>Kcnmb1<\/jats:italic>\n                    ; protein, BK-\u03b21) of the large Ca-activated K channel (BK) are hypertensive. This phenotype is thought to result from diminished BK currents in vascular smooth muscle where BK-\u03b21 is an ancillary subunit. However, the \u03b21-subunit is also expressed in the renal connecting tubule (CNT), a segment of the aldosterone-sensitive distal nephron, where it associates with BK and facilitates K secretion. Because of the correlation between certain forms of hypertension and renal defects, particularly in the distal nephron, it was determined whether the hypertension of Kcnmb1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    has a renal origin. We found that Kcnmb1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    are hypertensive, volume expanded, and have reduced urinary K and Na clearances. These conditions are exacerbated when the animals are fed a high K diet (5% K; HK). Supplementing HK-fed Kcnmb1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    with eplerenone (mineralocorticoid receptor antagonist) corrected the fluid imbalance and more than 70% of the hypertension. Finally, plasma [aldo] was elevated in Kcnmb1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    under basal conditions (control diet, 0.6% K) and increased significantly more than wild type when fed the HK diet. We conclude that the majority of the hypertension of Kcnmb1\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    is due to aldosteronism, resulting from renal potassium retention and hyperkalemia.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0904635106","type":"journal-article","created":{"date-parts":[[2009,6,25]],"date-time":"2009-06-25T21:59:42Z","timestamp":1245967182000},"page":"11800-11805","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":101,"title":["Hypertension of Kcnmb1\n                    <sup>\u2212\/\u2212<\/sup>\n                    is linked to deficient K secretion and aldosteronism"],"prefix":"10.1073","volume":"106","author":[{"given":"P. Richard","family":"Grimm","sequence":"first","affiliation":[{"name":"Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 985850 Nebraska Medical Center, Omaha, NE 68198-5850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Debra L.","family":"Irsik","sequence":"additional","affiliation":[{"name":"Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 985850 Nebraska Medical Center, Omaha, NE 68198-5850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deann C.","family":"Settles","sequence":"additional","affiliation":[{"name":"Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 985850 Nebraska Medical Center, Omaha, NE 68198-5850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. David","family":"Holtzclaw","sequence":"additional","affiliation":[{"name":"Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 985850 Nebraska Medical Center, Omaha, NE 68198-5850"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven. C.","family":"Sansom","sequence":"additional","affiliation":[{"name":"Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, 985850 Nebraska Medical Center, Omaha, NE 68198-5850"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2009,7,14]]},"reference":[{"key":"e_1_3_3_1_2","first-page":"1863","article-title":"Primary hyperaldosteronism in essential hypertensives: Prevalence, biochemical profile, and molecular biology","volume":"85","author":"Fardella CE","year":"2000","unstructured":"CE Fardella, et al., Primary hyperaldosteronism in essential hypertensives: Prevalence, biochemical profile, and molecular biology. J Clin Endocrinol Metab 85, 1863\u20131867 (2000).","journal-title":"J Clin Endocrinol Metab"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ajh.2008.234"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2265.2007.02775.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.83.11.1104"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.6.C1755"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI19580"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/35038011"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.87.11.e53"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.2001.91.3.1350"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00340.2004"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00118.2006"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.13.4237"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206644200"},{"key":"e_1_3_3_14_2","first-page":"F143","article-title":"Low-conductance K channels in apical membrane of rat cortical collecting tubule","volume":"256","author":"Frindt G","year":"1989","unstructured":"G Frindt, LG Palmer, Low-conductance K channels in apical membrane of rat cortical collecting tubule. Am J Physiol 256, F143\u2013F151 (1989).","journal-title":"Am J Physiol"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002438"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5002369"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajplegacy.1971.221.2.437"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0039-128X(93)90089-6"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1677\/joe.0.1170379"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107460"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.90527.2008"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-7793.1997.537bm.x"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00010.2003"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2007-0319"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(85)90423-7"},{"key":"e_1_3_3_26_2","first-page":"E752","article-title":"K+ channels in adrenal zona glomerulosa cells. I. Characterization of distinct channel types","volume":"263","author":"Vassilev PM","year":"1992","unstructured":"PM Vassilev, MV Kanazirska, SJ Quinn, DL Tillotson, GH Williams, K+ channels in adrenal zona glomerulosa cells. I. Characterization of distinct channel types. Am J Physiol 263, E752\u2013E759 (1992).","journal-title":"Am J Physiol"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.1.C239"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00169.2004"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M212301200"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ki.5000388"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.156968"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00002.2008"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00057.2005"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ASN.0000013292.78621.FD"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjhyper.2006.02.002"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(12)61380-9"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.0000156448.74296.FE"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.112619799"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-8993(90)90296-N"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/0167-4889(93)90164-K"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-129-4-2139"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-121-3-875"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-41-1-153"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-85-2-381"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-90-1-299"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20347"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000196557.93717.95"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddn168"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.88.2.210"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2004.068676"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00018.2007"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.1038\/ajh.2008.301"}],"container-title":["Proceedings of the National Academy of Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/pnas.org\/doi\/pdf\/10.1073\/pnas.0904635106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T17:31:06Z","timestamp":1649784666000},"score":1,"resource":{"primary":{"URL":"https:\/\/pnas.org\/doi\/full\/10.1073\/pnas.0904635106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,7,14]]},"references-count":52,"journal-issue":{"issue":"28","published-print":{"date-parts":[[2009,7,14]]}},"alternative-id":["10.1073\/pnas.0904635106"],"URL":"https:\/\/doi.org\/10.1073\/pnas.0904635106","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1161830.623445","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1161830.1123054","asserted-by":"object"},{"id-type":"doi","id":"10.3410\/f.1161830.625347","asserted-by":"object"}]},"ISSN":["0027-8424","1091-6490"],"issn-type":[{"value":"0027-8424","type":"print"},{"value":"1091-6490","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,7,14]]},"assertion":[{"value":"2009-02-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-07-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}