{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:56:44Z","timestamp":1790791004353,"version":"4.1.0"},"reference-count":32,"publisher":"National Academy of Sciences","issue":"33","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,8,18]]},"abstract":"<jats:p>\n                    Severe dengue infection in humans causes a disease characterized by thrombocytopenia, increased levels of cytokines, increased vascular permeability, hemorrhage, and shock. Treatment is supportive. Activation of platelet-activating factor (PAF) receptor (PAFR) on endothelial cells and leukocytes induces increase in vascular permeability, hypotension, and production of cytokines. We hypothesized that activation of PAFR could account for the major systemic manifestations of dengue infection. Inoculation of adult mice with an adapted strain of Dengue virus caused a systemic disease, with several features of the infection in humans. In PAFR\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    mice, there was decreased thrombocytopenia, hemoconcentration, decreased systemic levels of cytokines, and delay of lethality, when compared with WT infected mice. Treatment with UK-74,505, an orally active PAFR antagonist, prevented the above-mentioned manifestations, as well as hypotension and increased vascular permeability, and decreased lethality, even when started 5 days after virus inoculation. Similar results were obtained with a distinct PAFR antagonist, PCA-4246. Despite decreased disease manifestation, viral loads were similar (PAFR\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    ) or lower (PAFR antagonist) than in WT mice. Thus, activation of PAFR plays a major role in the pathogenesis of experimental dengue infection, and its blockade prevents more severe disease manifestation after infection with no increase in systemic viral titers, suggesting that there is no interference in the ability of the murine host to deal with the infection. PAFR antagonists are disease-modifying agents in experimental dengue infection.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0906467106","type":"journal-article","created":{"date-parts":[[2009,7,30]],"date-time":"2009-07-30T21:49:58Z","timestamp":1248990598000},"page":"14138-14143","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":110,"title":["Essential role of platelet-activating factor receptor in the pathogenesis of Dengue virus infection"],"prefix":"10.1073","volume":"106","author":[{"given":"Danielle G.","family":"Souza","sequence":"first","affiliation":[{"name":"Immunopharmacology, Departmento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"},{"name":"Departmento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caio T.","family":"Fagundes","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departmento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"},{"name":"Departmento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lirlandia P.","family":"Sousa","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departmento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Flavio A.","family":"Amaral","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departmento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"},{"name":"Departmento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rafael S.","family":"Souza","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departmento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"},{"name":"Departmento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adriano L.","family":"Souza","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departmento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erna G.","family":"Kroon","sequence":"additional","affiliation":[{"name":"Departmento de Microbiologia, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Sachs","sequence":"additional","affiliation":[{"name":"Immunopharmacology, Departmento de Bioquimica e Imunologia, Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Belo Horizonte, MG 31270-901, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernando Q.","family":"Cunha","sequence":"additional","affiliation":[{"name":"Departamento de Farmacologia, Faculdade de Medicina de Ribeir\u00e3o Preto, Universidade de S\u00e3o Paulo, Ribeir\u00e3o Preto 14049-900, Brazil;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eugenij","family":"Bukin","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology Safety, State Research Center of Virology and Biotechnology \u201cVector\u201d, Koltsovo, Novosibirsk Region, 630559 Russia; 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