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Gasp is a cytosolic protein with no known functional motifs that is expressed only in T cells, especially immature CD4\/CD8 double positive (DP) thymocytes. In the absence of Gasp, differentiation of both CD4 and CD8 single positive cells in the thymus was severely inhibited, whereas all other TCR-induced events such as \u03b2-selection, negative selection, peripheral activation, and homeostatic proliferation were unaffected. We found that Gasp constitutively associates with Grb2 via its N-terminal Src homology 3 domain, suggesting that Gasp acts as a thymocyte-specific adaptor for Grb2 or regulates Ras signaling in DP thymocytes. Collectively, we have described a gene called Gasp that is critical for positive selection.<\/jats:p>","DOI":"10.1073\/pnas.0908593106","type":"journal-article","created":{"date-parts":[[2009,9,14]],"date-time":"2009-09-14T21:48:54Z","timestamp":1252964934000},"page":"16345-16350","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":77,"title":["Gasp, a Grb2-associating protein, is critical for positive selection of thymocytes"],"prefix":"10.1073","volume":"106","author":[{"given":"Michael S.","family":"Patrick","sequence":"first","affiliation":[{"name":"Departments of aPathology and"},{"name":"Department of Microbiology, Yamaguchi University School of Medicine, 1-1-1 Minami-Kogushi, Ube, Yamaguchi 755-8505 Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyo","family":"Oda","sequence":"additional","affiliation":[{"name":"Departments of aPathology 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