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Other merits include: extremely low off rate once bound with target cells, rapid recognition ability with enhanced sensitivity, low critical micelle concentration values, and dual-drug delivery pathways. To prove the potential detection\/delivery application of this aptamer\u2013micelle in biological living systems, we mimicked a tumor site in the blood stream by immobilizing tumor cells onto the surface of a flow channel device. Flushing the aptamer\u2013micelles through the channel demonstrated their selective recognition ability under flow circulation in human whole-blood sample. The aptamer\u2013micelles show great dynamic specificity in flow channel systems that mimic drug delivery in the blood system. Therefore, our DNA aptamer\u2013micelle assembly has shown high potential for cancer cell recognition and for in vivo drug delivery applications.<\/jats:p>","DOI":"10.1073\/pnas.0909611107","type":"journal-article","created":{"date-parts":[[2009,12,22]],"date-time":"2009-12-22T21:09:29Z","timestamp":1261516169000},"page":"5-10","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":306,"title":["DNA aptamer\u2013micelle as an efficient detection\/delivery vehicle toward cancer cells"],"prefix":"10.1073","volume":"107","author":[{"given":"Yanrong","family":"Wu","sequence":"first","affiliation":[{"name":"Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at Bio\/Nano Interface, University of Florida Genetics Institute, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200; and"},{"name":"Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwame","family":"Sefah","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at Bio\/Nano Interface, University of Florida Genetics Institute, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200; and"},{"name":"Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haipeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at Bio\/Nano Interface, University of Florida Genetics Institute, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200; and"},{"name":"Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruowen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at Bio\/Nano Interface, University of Florida Genetics Institute, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200; and"},{"name":"Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weihong","family":"Tan","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Department of Physiology and Functional Genomics, Shands Cancer Center, Center for Research at Bio\/Nano Interface, University of Florida Genetics Institute, and McKnight Brain Institute, University of Florida, Gainesville, FL 32611-7200; and"},{"name":"Moffitt Cancer Center and Research Institute, 12902 Magnolia Drive, Tampa, FL 33612"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2009,12,22]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402028"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2009.04.010"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200603064"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/marc.200700779"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc010052t"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/adma.200700866"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2200121"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/346818a0"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602615103"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200502589"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja801339w"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac070189y"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M700026-MCP200"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707046105"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/48.10.1647"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkm358"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200600532"},{"key":"e_1_3_3_18_2","first-page":"2641","article-title":"Determinants of tumor blood flow-a review","volume":"48","author":"Jain RK","year":"1988","unstructured":"RK Jain, Determinants of tumor blood flow-a review. 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