{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:21:14Z","timestamp":1791300074925,"version":"4.1.0"},"reference-count":34,"publisher":"National Academy of Sciences","issue":"9","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,3,2]]},"abstract":"<jats:p>\n                    Cholera toxin is encoded in the genome of CTX\u03c6, a lysogenic filamentous phage of\n                    <jats:italic>Vibrio cholerae.<\/jats:italic>\n                    CTX\u03c6 variants contribute to the genetic diversity of cholera epidemic strains. It has been shown that the El Tor variant of CTX\u03c6 hijacks XerC and XerD, two host-encoded tyrosine recombinases that normally function to resolve chromosome dimers, to integrate at\n                    <jats:italic>dif<\/jats:italic>\n                    1, the dimer resolution site of the larger of the two\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    chromosomes. However, the exact mechanism of integration of CTX\u03c6 and the rules governing its integration remained puzzling, with phage variants integrated at either or both dimer resolution sites of the two\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    chromosomes. We designed a genetic system to determine experimentally the tropism of integration of CTX\u03c6 and thus define rules of compatibility between phage variants and dimer resolution sites. We then showed in vitro how these rules are explained by the direct integration of the single-stranded phage genome into the double-stranded bacterial genome. Finally, we showed how the evolution of phage attachment and chromosome dimer resolution sites contributes to the generation of genetic diversity among cholera epidemic strains.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0910212107","type":"journal-article","created":{"date-parts":[[2010,1,21]],"date-time":"2010-01-21T22:19:46Z","timestamp":1264112386000},"page":"4377-4382","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":65,"title":["Molecular keys of the tropism of integration of the cholera toxin phage"],"prefix":"10.1073","volume":"107","author":[{"given":"Bhabatosh","family":"Das","sequence":"first","affiliation":[{"name":"Centre de G\u00e9n\u00e9tique Mol\u00e9culaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France; and"},{"name":"Universit\u00e9 Paris-Sud, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien","family":"Bischerour","sequence":"additional","affiliation":[{"name":"Centre de G\u00e9n\u00e9tique Mol\u00e9culaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France; and"},{"name":"Universit\u00e9 Paris-Sud, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Eve","family":"Val","sequence":"additional","affiliation":[{"name":"Centre de G\u00e9n\u00e9tique Mol\u00e9culaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France; and"},{"name":"Universit\u00e9 Paris-Sud, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois-Xavier","family":"Barre","sequence":"additional","affiliation":[{"name":"Centre de G\u00e9n\u00e9tique Mol\u00e9culaire, Centre National de la Recherche Scientifique, 91198 Gif-sur-Yvette, France; and"},{"name":"Universit\u00e9 Paris-Sud, 91405 Orsay, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,1,21]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/1831533a0"},{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.272.5270.1910"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.24.6992-6998.2000"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907787106"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000201"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature00782"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/9781555817954.ch8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2004.04309.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.07.002"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-007-0275-0"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700365104"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.resmic.2007.11.015"},{"key":"e_1_3_3_13_2","article-title":"Cholera outbreaks caused by an altered Vibrio cholerae O1 El Tor biotype strain producing classical type cholera toxin B in Vietnam 2007-2008","author":"Minh NB","year":"2009","unstructured":"NB Minh, et al., Cholera outbreaks caused by an altered Vibrio cholerae O1 El Tor biotype strain producing classical type cholera toxin B in Vietnam 2007-2008. 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