{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:18:49Z","timestamp":1792660729949,"version":"4.5.0"},"reference-count":35,"publisher":"National Academy of Sciences","issue":"47","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2009,11,24]]},"abstract":"<jats:p>\n                    Phosphoinositide-specific phospholipase C (PLC) is a central effector for many biological responses regulated by G-protein\u2013coupled receptors including\n                    <jats:named-content content-type=\"genus-species\">Drosophila<\/jats:named-content>\n                    phototransduction where light sensitive channels are activated downstream of NORPA, a PLC\u03b2 homolog. Here we show that the sphingolipid biosynthetic enzyme, ceramide kinase, is a novel regulator of PLC signaling and photoreceptor homeostasis. A mutation in ceramide kinase specifically leads to proteolysis of NORPA, consequent loss of PLC activity, and failure in light signal transduction. The mutant photoreceptors also undergo activity-dependent degeneration. Furthermore, we show that a significant increase in ceramide, resulting from lack of ceramide kinase, perturbs the membrane microenvironment of phosphatidylinositol 4, 5, bisphosphate (PIP\n                    <jats:sub>2<\/jats:sub>\n                    ), altering its distribution. Fluorescence image correlation spectroscopic studies on model membranes suggest that an increase in ceramide decreases clustering of PIP\n                    <jats:sub>2<\/jats:sub>\n                    and its partitioning into ordered membrane domains. Thus ceramide kinase\u2013mediated maintenance of ceramide level is important for the local regulation of PIP\n                    <jats:sub>2<\/jats:sub>\n                    and PLC during phototransduction.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0911028106","type":"journal-article","created":{"date-parts":[[2009,11,5]],"date-time":"2009-11-05T21:40:29Z","timestamp":1257457229000},"page":"20063-20068","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":44,"title":["Ceramide kinase regulates phospholipase C and phosphatidylinositol 4, 5, bisphosphate in phototransduction"],"prefix":"10.1073","volume":"106","author":[{"given":"Ujjaini","family":"Dasgupta","sequence":"first","affiliation":[{"name":"Program in Gene Function and Expression and"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Bamba","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salvatore","family":"Chiantia","sequence":"additional","affiliation":[{"name":"Biotechnologisches Zentrum, Technical University of Dresden, 01187 Dresden, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pusha","family":"Karim","sequence":"additional","affiliation":[{"name":"Program in Gene Function and Expression and"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmad N. Abou","family":"Tayoun","sequence":"additional","affiliation":[{"name":"Department of Biology, Dartmouth College, Hanover, NH 03755;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikuko","family":"Yonamine","sequence":"additional","affiliation":[{"name":"Program in Gene Function and Expression and"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satinder S.","family":"Rawat","sequence":"additional","affiliation":[{"name":"Program in Gene Function and Expression and"},{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghavendra Pralhada","family":"Rao","sequence":"additional","affiliation":[{"name":"Laboratory of Cell and Developmental Signaling, National Cancer Institute, Frederick, MD 21702; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kunio","family":"Nagashima","sequence":"additional","affiliation":[{"name":"EM Facility\/Image Analysis Laboratory, SAIC, Frederick, MD 21702"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiichiro","family":"Fukusaki","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vishwajeet","family":"Puri","sequence":"additional","affiliation":[{"name":"Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick J.","family":"Dolph","sequence":"additional","affiliation":[{"name":"Department of Biology, Dartmouth College, Hanover, NH 03755;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Schwille","sequence":"additional","affiliation":[{"name":"Biotechnologisches Zentrum, Technical University of Dresden, 01187 Dresden, Germany;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jairaj K.","family":"Acharya","sequence":"additional","affiliation":[{"name":"Laboratory of Cell and Developmental Signaling, National Cancer Institute, Frederick, MD 21702; 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