{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T12:55:18Z","timestamp":1793278518926,"version":"4.5.0"},"reference-count":53,"publisher":"National Academy of Sciences","issue":"7","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,2,16]]},"abstract":"<jats:p>\n                    The upper respiratory tract is continually assaulted with harmful dusts and xenobiotics carried on the incoming airstream. Detection of such irritants by the trigeminal nerve evokes protective reflexes, including sneezing, apnea, and local neurogenic inflammation of the mucosa. Although free intra-epithelial nerve endings can detect certain lipophilic irritants (e.g., mints, ammonia), the epithelium also houses a population of trigeminally innervated solitary chemosensory cells (SCCs) that express T2R bitter taste receptors along with their downstream signaling components. These SCCs have been postulated to enhance the chemoresponsive capabilities of the trigeminal irritant-detection system. Here we show that transduction by the intranasal solitary chemosensory cells is necessary to evoke trigeminally mediated reflex reactions to some irritants including acyl\u2013homoserine lactone bacterial quorum-sensing molecules, which activate the downstream signaling effectors associated with bitter taste transduction. Isolated nasal chemosensory cells respond to the classic bitter ligand denatonium as well as to the bacterial signals by increasing intracellular Ca\n                    <jats:sup>2<\/jats:sup>\n                    <jats:sup>+<\/jats:sup>\n                    . Furthermore, these same substances evoke changes in respiration indicative of trigeminal activation. Genetic ablation of either G\u03b1-gustducin or TrpM5, essential elements of the T2R transduction cascade, eliminates the trigeminal response. Because acyl\u2013homoserine lactones serve as quorum-sensing molecules for Gram-negative pathogenic bacteria, detection of these substances by airway chemoreceptors offers a means by which the airway epithelium may trigger an epithelial inflammatory response before the bacteria reach population densities capable of forming destructive biofilms.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0911934107","type":"journal-article","created":{"date-parts":[[2010,1,26]],"date-time":"2010-01-26T23:49:26Z","timestamp":1264549766000},"page":"3210-3215","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":387,"title":["Nasal chemosensory cells use bitter taste signaling to detect irritants and bacterial signals"],"prefix":"10.1073","volume":"107","author":[{"given":"Marco","family":"Tizzano","sequence":"first","affiliation":[{"name":"Rocky Mountain Taste and Smell Center,"},{"name":"Department of Cell and Developmental Biology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian D.","family":"Gulbransen","sequence":"additional","affiliation":[{"name":"Rocky Mountain Taste and Smell Center,"},{"name":"Department of Cell and Developmental Biology,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aurelie","family":"Vandenbeuch","sequence":"additional","affiliation":[{"name":"Rocky Mountain Taste and Smell Center,"},{"name":"Department of Otolaryngology, and"},{"name":"Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tod R.","family":"Clapp","sequence":"additional","affiliation":[{"name":"Rocky Mountain Taste and Smell Center,"},{"name":"Department of Biomedical Sciences, Colorado State University, Fort Collins, CO 80523; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jake P.","family":"Herman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiruy M.","family":"Sibhatu","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Colorado Denver, Aurora, CO 80045,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mair E. 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