{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T07:01:56Z","timestamp":1791615716608,"version":"4.3.4"},"reference-count":36,"publisher":"National Academy of Sciences","issue":"12","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,3,23]]},"abstract":"<jats:p>\n                    Community-associated methicillin-resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (CA-MRSA) is epidemic in the United States, even rivaling HIV\/AIDS in its public health impact. The pandemic clone USA300, like other CA-MRSA strains, expresses Panton-Valentine leukocidin (PVL), a pore-forming toxin that targets polymorphonuclear leukocytes (PMNs). PVL is thought to play a key role in the pathogenesis of necrotizing pneumonia, but data from rodent infection models are inconclusive. Rodent PMNs are less susceptible than human PMNs to PVL-induced cytolysis, whereas rabbit PMNs, like those of humans, are highly susceptible to PVL-induced cytolysis. This difference in target cell susceptibility could affect results of experimental models. Therefore, we developed a rabbit model of necrotizing pneumonia to compare the virulence of a USA300 wild-type strain with that of isogenic PVL-deletion mutant and -complemented strains. PVL enhanced the capacity of USA300 to cause severe lung necrosis, pulmonary edema, alveolar hemorrhage, hemoptysis, and death, hallmark clinical features of fatal human necrotizing pneumonia. Purified PVL instilled directly into the lung caused lung inflammation and injury by recruiting and lysing PMNs, which damage the lung by releasing cytotoxic granule contents. These findings provide insights into the mechanism of PVL-induced lung injury and inflammation and demonstrate the utility of the rabbit for studying PVL-mediated pathogenesis.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0912403107","type":"journal-article","created":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T23:59:34Z","timestamp":1268697574000},"page":"5587-5592","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":316,"title":["Polymorphonuclear leukocytes mediate\n                    <i>Staphylococcus aureus<\/i>\n                    Panton-Valentine leukocidin-induced lung inflammation and injury"],"prefix":"10.1073","volume":"107","author":[{"given":"Binh An","family":"Diep","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, CA 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Lyon, France;"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U851, Centre National de R\u00e9f\u00e9rence des Staphylocoques, 69008 Lyon, France;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome","family":"Etienne","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine Laennec, Universit\u00e9 Lyon 1, 69008 Lyon, France;"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U851, Centre National de R\u00e9f\u00e9rence des Staphylocoques, 69008 Lyon, France;"},{"name":"Hospices Civils de Lyon, Centre de Biologie Est, 69500 Lyon, France; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard","family":"Lina","sequence":"additional","affiliation":[{"name":"Facult\u00e9 de M\u00e9decine Laennec, Universit\u00e9 Lyon 1, 69008 Lyon, France;"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U851, Centre National de R\u00e9f\u00e9rence des 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