{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T15:15:54Z","timestamp":1790867754772,"version":"4.1.0"},"reference-count":39,"publisher":"National Academy of Sciences","issue":"19","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,5,11]]},"abstract":"<jats:p>\n                    Cholera and enterotoxigenic\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    (ETEC) are among the most common causes of acute infantile gastroenteritis globally. We previously developed a rice-based vaccine that expressed cholera toxin B subunit (MucoRice-CTB) and had the advantages of being cold chain\u2013free and providing protection against cholera toxin (CT)\u2013induced diarrhea. To advance the development of MucoRice-CTB for human clinical application, we investigated whether the CTB-specific secretory IgA (SIgA) induced by MucoRice-CTB gives longstanding protection against diarrhea induced by\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    and heat-labile enterotoxin (LT)\u2013producing ETEC (LT-ETEC) in mice. Oral immunization with MucoRice-CTB stored at room temperature for more than 3 y provided effective SIgA-mediated protection against CT- or LT-induced diarrhea, but the protection was impaired in polymeric Ig receptor\u2013deficient mice lacking SIgA. The vaccine gave longstanding protection against CT- or LT-induced diarrhea (for \u22656 months after primary immunization), and a single booster immunization extended the duration of protective immunity by at least 4 months. Furthermore, MucoRice-CTB vaccination prevented diarrhea in the event of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    and LT-ETEC challenges. Thus, MucoRice-CTB is an effective long-term cold chain\u2013free oral vaccine that induces CTB-specific SIgA-mediated longstanding protection against\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    \u2013 or LT-ETEC\u2013induced diarrhea.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0914121107","type":"journal-article","created":{"date-parts":[[2010,4,26]],"date-time":"2010-04-26T21:49:06Z","timestamp":1272318546000},"page":"8794-8799","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":108,"title":["Secretory IgA-mediated protection against\n                    <i>V. cholerae<\/i>\n                    and heat-labile enterotoxin-producing enterotoxigenic\n                    <i>Escherichia coli<\/i>\n                    by rice-based vaccine"],"prefix":"10.1073","volume":"107","author":[{"given":"Daisuke","family":"Tokuhara","sequence":"first","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshikazu","family":"Yuki","sequence":"additional","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonori","family":"Nochi","sequence":"additional","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshio","family":"Kodama","sequence":"additional","affiliation":[{"name":"Department of Bacterial Infections, Research Institute for Microbial Diseases, Osaka University, Osaka 565-0871, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mio","family":"Mejima","sequence":"additional","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiho","family":"Kurokawa","sequence":"additional","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuko","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Division of Mucosal Immunology, Department of Microbiology and Immunology, The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masanobu","family":"Nanno","sequence":"additional","affiliation":[{"name":"Yakult Central Institute for Microbiological Research, Tokyo 186-8650, Japan; 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