{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:32:05Z","timestamp":1791253925524,"version":"4.1.0"},"reference-count":59,"publisher":"National Academy of Sciences","issue":"8","content-domain":{"domain":["www.pnas.org"],"crossmark-restriction":true},"short-container-title":["Proc. Natl. Acad. Sci. U.S.A."],"published-print":{"date-parts":[[2010,2,23]]},"abstract":"<jats:p>\n                    Nuclear factor of activated T cell (NFAT) transcription factors are key regulators of gene transcription within immune cells. The NFAT-interacting protein, (NIP45), augments NFAT-driven IL-4 expression by a mechanism that relies on arginine methylation. To establish the function of NIP45 in vivo, we generated mice with a targeted deletion of the gene encoding this cofactor.\n                    <jats:italic>NIP45<\/jats:italic>\n                    -deficient T helper cells displayed profound defects in the expression of NFAT-regulated cytokine genes, including IL-4. Whereas\n                    <jats:italic>NIP45<\/jats:italic>\n                    deficiency does not interfere with T helper cell NFAT activation or lineage-specific transcription-factor expression, NIP45 acts as an enhancer for the assembly of protein arginine methyltransferase 1 and the protein arginine methyltransferase 1-linked histone 4 arginine 3 methylation with the IL-4 promoter. Our study reveals an essential role for NIP45 in promoting robust cytokine expression in vivo, which is required for the efficient handling of parasites. We propose that NIP45 acts as a molecular rheostat serving to amplify the type-2 immune response.\n                  <\/jats:p>","DOI":"10.1073\/pnas.0914700107","type":"journal-article","created":{"date-parts":[[2010,2,4]],"date-time":"2010-02-04T22:34:56Z","timestamp":1265322896000},"page":"3663-3668","update-policy":"https:\/\/doi.org\/10.1073\/pnas.cm10313","source":"Crossref","is-referenced-by-count":24,"title":["NIP45 controls the magnitude of the type 2 T helper cell response"],"prefix":"10.1073","volume":"107","author":[{"given":"John W.","family":"Fathman","sequence":"first","affiliation":[{"name":"Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael F.","family":"Gurish","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Immunology, and Allergy, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA 02115;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"Hemmers","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Bonham","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel S.","family":"Friend","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA 02115; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"Grusby","sequence":"additional","affiliation":[{"name":"Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie H.","family":"Glimcher","sequence":"additional","affiliation":[{"name":"Department of Medicine, Division of Rheumatology, Immunology, and Allergy, Brigham and Women\u2019s Hospital and Harvard Medical School, Boston, MA 02115;"},{"name":"Department of Immunology and Infectious Diseases, Harvard School of Public Health, Boston, MA 02115"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerri A.","family":"Mowen","sequence":"additional","affiliation":[{"name":"Department of Chemical Physiology, The Scripps Research Institute, La Jolla, CA 92037;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"341","published-online":{"date-parts":[[2010,2,23]]},"reference":[{"key":"e_1_3_3_1_2","doi-asserted-by":"crossref","unstructured":"D Yu et al. 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